Back to results
US 9,018,584 B2Granted
Devices and methods for infrared (IR) based quantitation of biomolecules
AssigneeEMD Millipore Corporation
InventorsElena Chernokalskaya, Vivek Joshi, Phillip Clark, Christopher Utzat, Ryan Amara, Timothy Scott Rider
Life of this patent
Estimating the term…
Abstract
The present invention provides methods for quantitating one or more biomolecules in a sample using IR based techniques, sample holder devices for use in such methods as well as methods for manufacturing such sample holder devices.
Claims24
A method for quantitation of one or more biomolecules in a sample, the method comprising the steps of:
- (a) providing a sample holder comprising a porous membrane which comprises a hydrophilic region surrounded by a hydrophobic region for sample containment;
- (b) contacting the hydrophilic region of the membrane with a sample volume, wherein the hydrophilic region has a diameter;
- (c) drying the sample volume on the membrane;
- (d) exposing the sample volume on the membrane to an infrared beam having a diameter equal to or larger than the diameter of the hydrophilic region, and comprising a wavelength in the spectral range of 4000-400 cm−1 or any portion of the spectral range of 4000-400 cm−1, thereby to obtain an infrared absorption spectrum;
- wherein one or more absorption peak areas in the infrared absorption spectrum correlates with the quantity of the one or more biomolecules in the sample.
2 more elements
A method for quantitation of one or more biomolecules in a sample, the method comprising the steps of:
- (a) providing a sample holder card comprising a porous membrane which comprises a hydrophilic region surrounded by a hydrophobic region for sample containment;
- (b) contacting the hydrophilic region of the membrane with a sample volume;
- (c) drying the sample volume on the membrane;
- (d) detecting the presence of water on the membrane using infrared absorbance and repeating step (c), if necessary, until no water is detected;
- and
- (e) exposing the sample volume on the membrane to an infrared beam comprising a wavelength in the spectral range of 4000-400 cm−1 or any portion of the spectral range, thereby to obtain an infrared, absorption spectrum;
- wherein one or more absorption peak areas in the infrared absorption spectrum correlates with the quantity of the one or more biomolecules in the sample.
4 more elements
Dependencies are inferred from the claim text.