Isolation and purification of antibodies using protein a affinity chromatography
AssigneeAbbVie Inc.
InventorsRobert K. Hickman, Qing Huang, Cheryl L. Weed, Scott T. Ennis, Barbara Perilli-Palmer, Min Wan
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Abstract
Disclosed herein are methods for the isolation and purification of antibodies wherein the use of an affinity chromatographic step results in an antibody composition sufficiently pure for pharmaceutical uses. The methods described herein comprise pH viral reduction/inactivation, ultrafiltration/diafiltration, affinity chromatography, preferably Protein A affinity, ion exchange chromatography, and hydrophobic chromatography. Further, the present invention is directed toward pharmaceutical compositions comprising one or more antibodies of the present invention.
Claims13
A process for purifying adalimumab from a fermentation harvest of a Chinese Hamster Ovary (CHO) cell culture expressing said adalimumab, said process comprising:
- a) binding adalimumab from said fermentation harvest to a Protein A resin,
- b) eluting the bound adalimumab at an elution pH of 3.6-4, and
- c) incubating the eluted adalimumab for 1 to 3 hours.
Dependencies are inferred from the claim text.