Stable Tregs and related materials and methods
AssigneeThe University of Toledo
InventorsStanislaw M. Stepkowski, Wenhao Chen, Yoshihiro Miyahara
Life of this patent
Estimating the term…
Abstract
The present invention provides materials and methods related to culturing stable Treg cells or in vivo expansion of stable Treg cells, the cells made by the methods, treatments for various inflammatory/autoimmune pathologies and transplant/graft rejection, and related materials. Ex vivo induction and expansion of the stable Tregs is described, including use of inducing compositions, such as certain mAbs and other compounds, along with expansion medium comprising IL-2. In vivo expansion of stable Treg cells and treatments for various inflammatory/autoimmune pathologies and transplant/graft rejection are described, including the use of mAbs and their variants.
Claims3
A method to make stable iTreg cells, comprising introducing an inducer composition to an inducible first cell culture,
- wherein the inducer composition comprises anti-CD3 mAb/anti-CD28 mAb and at least one composition selected from the group consisting of:
- anti-IL-2;
- antiCD25 mAb;
- or Janus tyrosine kinase (Jak3) inhibitor, and
- wherein the first cell culture comprises CD4+Foxp3/GFP− cells in a medium with or without syngeneic antigen presenting cells.
Dependencies are inferred from the claim text.