Recombinant bacteria comprising novel sucrose transporters
AssigneeE.I. du Pont de Nemours and Company
InventorsKristin Ruebling-Jass, Jean-Francois Tomb, Tina K Van Dyk, Zheng You
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Abstract
Recombinant bacteria capable of metabolizing sucrose are described. The recombinant bacteria comprise in their genome or on at least one recombinant construct, a novel nucleotide sequence encoding a polypeptide having sucrose transporter activity and a nucleotide sequence encoding a polypeptide having sucrose hydrolase activity. These nucleotide sequences are each operably linked to the same or a different promoter. Recombinant bacteria capable of metabolizing sucrose to produce glycerol and/or glycerol-derived products such as 1,3-propanediol and 3-hydroxypropionic acid are also described.
Claims6
A recombinant bacterium comprising in its genome or in at least one recombinant construct:
- (a) a nucleotide sequence encoding a polypeptide having sucrose transporter activity, said polypeptide having at least 95% sequence identity, based on a Clustal W method of alignment, when compared to an amino acid sequence selected from the group consisting of SEQ ID NO:78 and SEQ ID NO:82;
- and
- (b) a nucleotide sequence encoding a polypeptide having sucrose hydrolase catalytic activity, wherein the polypeptide having sucrose hydrolase catalytic activity is classified as EC 3.2.1.26 or EC 2.4.1.7;
- wherein (a) and (b) are each operably linked to the same or a different promoter, wherein each of (a) and (b):
- (i) is in a recombinant construct;
- (ii) encodes a polypeptide comprising a non-native polypeptide;
- (iii) is a foreign gene;
- or (iv) is any combination of (i), (ii), or (iii), and further wherein said recombinant bacterium is capable of metabolizing sucrose.
Dependencies are inferred from the claim text.