Prostasin partial peptide and anti-prostasin antibody
AssigneesHitachi Chemical Company, Ltd., Kimio Tomita, Kenichiro Kitamura
InventorsHiromi Iijima, Noriya Ohta, Kimio Tomita, Kenichiro Kitamura
Life of this patent
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Abstract
An object of the present invention is to provide an antibody that can be stably supplied and can react with prostasin under non-denaturation and denaturation conditions, and an antigen peptide for preparation of the antibody. The present invention relates to a peptide consisting of the amino acid sequence shown in SEQ ID NO: 1 or a peptide consisting of an amino acid sequence that has a deletion, a substitution, or an addition of one or several amino acids with respect to the amino acid sequence shown in SEQ ID NO: 1 and having antigenicity of prostasin. Furthermore, the present invention relates to an antibody prepared using the peptide as an antigen.
Claims34
A peptide consisting of an amino acid sequence that has a deletion, a substitution, or an addition consisting of one to five amino acids with respect to the amino acid sequence shown in SEQ ID NO:
- 1, and having antigenicity of prostasin.
A peptide having a label bound thereto, the peptide being the peptide consisting of the amino acid sequence shown in SEQ ID NO:
- 1.
A peptide having a carrier protein bound thereto, the peptide being the peptide consisting of the amino acid sequence shown in SEQ ID NO:
- 1.
An antibody, which is prepared using a peptide consisting of the amino acid sequence shown in SEQ ID NO:
- 1 as an antigen, wherein the antibody reacts with the peptide and/or specifically binds to a prostasin under denaturation and non-denaturation conditions, and wherein the antibody is a monoclonal antibody.
A reagent for an immunological assay for prostasin, comprising:
- (a) an antibody, which is prepared using a peptide consisting of the amino acid sequence shown in SEQ ID NO:
- 1 as an antigen, wherein the antibody reacts with the peptide and/or specifically binds to a prostasin under denaturation and non-denaturation conditions;
- and
- (b) a peptide consisting of the amino acid sequence shown in SEQ ID NO:
- 1, which is used as a competitive substance in a competitive immunoassay for prostasin.
A method for detecting prostasin in a sample, comprising the steps of:
- (a) contacting an antibody with a sample;
- and
- (b) detecting whether or not the antibody binds to prostasin in the sample,
- wherein the antibody is prepared using a peptide consisting of the amino acid sequence shown in SEQ ID NO:
- 1 as an antigen,
- wherein the antibody reacts with the peptide and/or specifically binds to a prostasin under denaturation and non-denaturation conditions, and
- wherein a peptide consisting of the amino acid sequence shown in SEQ ID NO:
- 1, which is used as a competitive substance in a competitive immunoassay for prostasin, is added as a competitive substance in step (a).
A method for detecting prostasin in a sample, comprising the steps of:
- (a) contacting an antibody with a sample;
- and
- (b) detecting whether or not the antibody binds to prostasin in the sample,
- wherein the antibody is prepared using a peptide consisting of the amino acid sequence shown in SEQ ID NO:
- 1 as an antigen,
- wherein the antibody reacts with the peptide and/or specifically binds to a prostasin under denaturation and non-denaturation conditions, and
- wherein the antibody is bound to a solid phase.
A method for producing an antibody against prostasin, comprising the steps of:
- (a) preparing (i) a peptide consisting of the amino acid sequence shown in SEQ ID NO:
- 1, or (ii) a peptide consisting of an amino acid sequence that has a deletion, a substitution, or an addition consisting of not more than one to five amino acids with respect to the amino acid sequence shown in SEQ ID NO:
- 1, and having antigenicity of prostasin;
- (b) administering the peptide to an animal;
- and
- (c) collecting the antibody against the peptide from the animal.
A method for producing an antibody against prostasin, comprising the steps of:
- (a) preparing (i) a peptide consisting of the amino acid sequence shown in SEQ ID NO:
- 1, or (ii) a peptide consisting of an amino acid sequence that has a deletion, a substitution, or an addition consisting of not more than one to five amino acids with respect to the amino acid sequence shown in SEQ ID NO:
- 1, and having antigenicity of prostasin;
- (b) administering the peptide to an animal;
- (c) collecting antibody-producing cells from the animal;
- (d) fusing the antibody-producing cells with myeloma cells to obtain hybridomas;
- and
- (e) collecting then antibody against the peptide from the hybridomas.
Dependencies are inferred from the claim text.