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US 9,017,947 B2Granted
Soybean event MON89788 and methods for detection thereof
AssigneeMonsanto Technology LLC
InventorsMarianne Malven, Jennifer Rinehart, Nancy Taylor, Ellen Dickinson
Life of this patent
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Abstract
The present invention provides for soybean plant and seed comprising transformation event MON89788 and DNA molecules unique to these events. The invention also provides methods for detecting the presence of these DNA molecules in a sample.
Claims5
A method of detecting the presence of DNA corresponding to the soybean event MON89788 in a sample, the method comprising:
- (a) contacting a sample comprising soybean DNA with a primer set, wherein a first DNA molecule of the primer set comprises at least 11 contiguous nucleotides of SEQ ID NO:3, SEQ ID NO:4 or a full complement thereof to function when used in a nucleic acid amplification reaction with DNA from soybean event MON89788 to produce a diagnostic amplicon for soybean event MON89788, wherein the diagnostic amplicon comprises SEQ ID NO:1, SEQ ID NO:2, or a full complement thereof;
- and
- (b) performing a nucleic acid amplification reaction, thereby producing the diagnostic amplicon;
- and
- (c) detecting the diagnostic amplicon.
2 more elements
A method of detecting the presence of a nucleic acid corresponding to event MON89788 in a sample, the method comprising:
- (a) obtaining a sample of soybean DNA;
- (b) contacting the sample with a DNA probe comprising a nucleotide sequence of sufficient length of contiguous nucleotides of SEQ ID NO:3, SEQ ID NO:4, or a full complement thereof, to function as a DNA probe that hybridizes under stringent hybridization conditions with a DNA molecule comprising a nucleotide sequence of SEQ ID NO:1 or SEQ ID NO:2 and does not hybridize under stringent hybridization conditions with a DNA molecule not comprising SEQ ID NO:1 or SEQ ID NO:2;
- (c) subjecting the sample and DNA probe to stringent hybridization conditions;
- and
- (d) detecting hybridization of the DNA probe to DNA in the sample, wherein detection of hybridization indicates the presence of a DNA molecule from a transgenic soybean plant comprising event MON89788.
2 more elements
Dependencies are inferred from the claim text.