Bacterial extract for digestive or urinary tract disorders and process for its preparation

A method of stimulating an immune response in a subject, comprising administering to said subject, an effective amount of an extract obtained from one or more of Escherichia coli (E. coli) bacterial strains selected from NCTC:

• 8603, 8621, 8622, 8623, 9026, 9111, 9119, 9707, and 9708 and I:
• 081, 082, 083, 084, 085, 086, 087, 088, and 089, lysed at an initial pH greater than 12, wherein the individual bacterial strains are grown in a medium which does not pose risk of prion diseases, wherein the extract is treated so as to reduce the nucleic acids to less than 100 μg/ml, wherein said extract comprises one or more amino acids, and wherein 3-80% of said one or more amino acids is racemized from L to D, and said one or more amino acids is chosen from aspartic acid, asparagine, glutamic acid, glutamine, serine, methionine, histidine, alanine, arginine, phenylalanine, tyrosine, leucine, lysine, valine, and threonine, wherein said extract preserves and/or retains saccharide components from Escherichia coli (E. coli).