Aptamer for chymase, and use thereof
AssigneesRibomic Inc., Otsuka Pharmaceutical Co., Ltd.
InventorsYoshikazu Nakamura, Ling Jin, Satoko Yamazaki, Hisako Ikeda, Masahiro Muraguchi
Life of this patent
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Abstract
Provided are an aptamer bound to chymase to inhibit activity of chymase; an aptamer containing a nucleotide sequence represented by X1GAUAGAN1N2UAAX2 wherein X1 and X2 are the same or different and each is A or G, and N1 and N2 are the same or different and each is A, G, C, U or T; a complex containing the aptamer and a functional substance (e.g., affinity substance, labeling substance, enzyme, drug delivery medium, drug and the like); a pharmaceutical or a reagent containing the aptamer or complex; a detection and a purification methods of chymase using the aptamer or complex and the like.
Claims21
An aptamer that binds to chymase to inhibit a chymase activity, comprising a nucleotide sequence represented by X1GAUAGAN1N2UAAX2 (SEQ ID NO:
- 21) wherein each of X1 and X2, whether identical or not, is A or G, and each of N1 and N2, whether identical or not, is A, G, C, U or T, and
- wherein the fourth nucleotide from the 5′ end is a 2′-fluoro-modified uracil.
An aptamer that binds to chymase to inhibit a chymase activity, comprising any one of the nucleotide sequences (a), (b) and (c) below:
- (a) an aptamer comprising a nucleotide sequence selected from among SEQ ID NO:
- 4-20, 22-34, 38-57, 59-65 and 69-72 with the provision that the uracil may be thymine;
- (b) an aptamer comprising a nucleotide sequence selected from among SEQ ID NO:
- 4-20, 22-34, 38-57, 59-65 and 69-72 with the provision that the uracil may be thymine, wherein 1 to 5 nucleotides are substituted, deleted, inserted or added;
- and
- (c) a nucleotide sequence having an identity of 70% or more to a nucleotide sequence selected from among SEQ ID NO:
- 4-20, 22-34, 38-57, 59-65 and 69-72 with the provision that the uracil may be thymine.
An aptamer that binds to chymase to inhibit a chymase activity, comprising any one of the nucleotide sequences (a′), (b′) and (c′) below:
- (a′) a nucleotide sequence selected from among SEQ ID NOs:
- 73-131 with the provision that the uracil may be thymine;
- (b′) a nucleotide sequence selected from among SEQ ID NOs:
- 73-131 with the provision that the uracil may be thymine, wherein 1 to 5 nucleotides are substituted, deleted, inserted or added;
- and
- (c′) a nucleotide sequence having an identity of 70% or more to a nucleotide sequence selected from among SEQ ID NOs:
- 73-131 with the provision that the uracil may be thymine).
Dependencies are inferred from the claim text.