Thyroglobulin quantitation by mass spectroscopy
AssigneeQuest Diagnostics Investments Incorporated
InventorsYanni Zhang, Nigel J. Clarke, Richard E. Reitz
Life of this patent
Estimating the term…
Abstract
Provided are methods for determining the amount of thyroglobulin in a sample using various purification steps followed by mass spectrometry. The methods generally involve purifying thyroglobulin in a test sample, digesting thyroglobulin to form peptide T129, purifying peptide T129, ionizing peptide T129, detecting the amount of peptide T129 ion generated, and relating the amount of peptide T129 ion to the amount of thyroglobulin originally present in the sample.
Claims13
A method for determining the amount of thyroglobulin in a test sample, comprising:
- (a) digesting thyroglobulin (Tg) and an added isotopically labeled thyroglobulin peptide standard in said test sample to form Tg peptides and Tg peptide standard products, wherein the Tg peptide standard has an amino acid sequence VIFDANAPVAVR (SEQ ID NO:1), wherein one or more valine is isotopically labeled with 13C, 15N;
- (b) purifying said Tg peptides and Tg peptide standard products from step (a);
- (c) ionizing said Tg peptides and Tg peptide standard products from step (b) to produce one or more Tg peptide ions and Tg peptide standard product ions detectable by mass spectrometry;
- and
- (d) detecting the amount of the ion(s) from step (c) by mass spectrometry;
- wherein the amount of the ion(s) detected in step (d) is related to the amount of thyroglobulin in said test sample and the amount of thyroglobulin peptide standard that was digested in step (a).
A method for determining the amount of thyroglobulin in a test sample, comprising:
- (a) digesting thyroglobulin in said test sample to form peptide T129 and digesting an added isotopically labeled thyroglobulin peptide standard in said test sample to form an isotopically labeled peptide T129 internal standard, wherein the T129 internal standard has an amino acid sequence VIFDANAPVAVR (SEQ ID NO:1), wherein one or more valine is isotopically labeled with 13C,15N;
- (b) purifying said peptide T129 and peptide T129 internal standard from step (a);
- (c) ionizing said peptide T129 and peptide T129 internal standard from step (b) to generate two or more precursor ions detectable by tandem mass spectrometry;
- wherein said precursor ion to peptide T129 has a mass/charge ratio of 636.4±0.5;
- (d) fragmenting said precursor ions in said mass spectrometric instrument to generate one or more fragment ions detectable by mass spectrometry, wherein one or more of said fragment ions of peptide T129 are selected from the list of ions having a mass/charge ratio of 797.4±0.5, 912.4±0.5, or 1059.5±0.5;
- and
- (f) detecting the amount of said precursor ions of step (d), one or more of said fragment ions of step (e), or both, by mass spectrometry;
- wherein the amount of ion(s) detected in step (f) is related to the amount of said thyroglobulin in said test sample.
Dependencies are inferred from the claim text.