Production of hemagglutinin-neuraminidase protein in microalgae
AssigneesMerial Limited, Sanofi Vaccine Technologies, S.A.S.
InventorsAnne-Cécile V. Bayne, James Casey Lippmeier, Kirk Emil Apt, Xuan Guo, Joyce A. Pritchard
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Abstract
The present invention is directed to recombinant microalgal cells and their use in production of heterologous hemagglutinin-neuraminidase (HN) polypeptides, as well as compositions and uses thereof.
Claims12
A method for production of a hemagglutinin-neuraminidase (HN) polypeptide, wherein said HN polypeptide is glycosylated and comprises full length HN polypeptide, a membrane domain and a native HN signal peptide,
- said method comprising culturing a recombinant microalgal host cell in a medium, wherein the recombinant microalgal host cell comprises a heterologous nucleic acid molecule comprising the sequence of SEQ ID NO:1 encoding the HN polypeptide to produce the HN polypeptide, and wherein the microalgal host cell is free of polynucleotide sequences encoding other heterologous viral polypeptides, whereby the HN polypeptide is secreted into the medium by action of the signal peptide, and
- recovering the HN polypeptide from the medium.
A method of producing a composition comprising a heterologous HN polypeptide, the method comprising:
- (a) expressing a heterologous HN polypeptide encoded by a polynucleotide comprising the sequence of SEQ ID NO:1 in a microalgal host cell, wherein said HN polypeptide is glycosylated and comprises full length HN polypeptide, a membrane domain and a native HN signal peptide, and
- (b) culturing the microalgal host cell under conditions sufficient to produce the heterologous FN polypeptide
- wherein the composition is produced as the culture supernatant comprising the heterologous FN polypeptide, and wherein the microalgal host cell is free of polynucleotide sequences encoding other heterologous viral polypeptides.
A recombinant microalgal cell expressing an HN polypeptide, wherein said HN polypeptide is glycosylated and comprises full length HN polypeptide, a membrane domain and a native HN signal peptide, comprising a nucleic acid molecule comprising a polynucleotide sequence of SEQ ID NO:1 that encodes the HN protein, wherein the microalgal cell is free of polynucleotide sequence encoding other heterologous viral proteins.
Dependencies are inferred from the claim text.