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US 9,012,176 B2Granted
Chinese hamster ovary cell lines
AssigneeAgency for Science, Technology and Research (A*Star)
InventorZhiwei Song
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Abstract
We provide a Chinese Hamster Ovary (CHO) cell which is capable of higher protein sialylation compared to a wild type Chinese Hamster Ovary cell, such as in the presence of functional GnT 1, in which the CHO cell is obtainable by selection with Ricinus communis agglutinin I (RCA-I).
Claims12
A method of expressing a protein of interest from a Chinese Hamster Ovary (CHO) cell, the method comprising:
- (a) introducing a nucleic acid encoding a protein of interest into a mutant Chinese Hamster Ovary (CHO) cell, wherein said mutant CHO cell comprises:
- (i) a loss of function mutation in the N-Acetyl-glucosaminyltransferase I (GnT 1) gene, and (ii) an exogenous nucleic acid sequence encoding functional GnT I;
- and
- (b) allowing the protein of interest to be expressed from the mutant CHO cell or a descendant thereof;
- and
- (c) purifying the protein.
3 more elements
Dependencies are inferred from the claim text.