PCV2 immunogenic compositions and methods of producing such compositions

A method of providing a protective effect against clinical symptoms of PCV2 infection in a pig after administration of a single dose of an immunogenic composition comprising the step of:

• administering said immunogenic composition to said pig, wherein said immunogenic composition comprises an effective amount of recombinant PCV2 ORF2 protein selected from the group consisting of:
• i) a polypeptide comprising a sequence selected from the group consisting of SEQ ID NO:
• 5, SEQ ID NO:
• 6, SEQ ID NO:
• 9, SEQ ID NO:
• 10, and SEQ ID NO:
• 11;
• ii) any polypeptide that is at least 90% homologous to the polypeptide of i);
• iii) a polypeptide that is encoded by a DNA comprising the sequence of SEQ ID NO:
• 3 or SEQ ID NO:
• 4;
• and
• iv) any polypeptide that is encoded by a polynucleotide that is at least 90% homologous to the polynucleotide of iii.

An immunogenic composition comprising:

• an effective amount of recombinant PCV2 ORF2 protein and a carrier, wherein said PCV2 ORF2 protein is selected from the group consisting of:
• i) a polypeptide comprising a sequence selected from the group consisting of SEQ ID NO:
• 5, SEQ ID NO:
• 6, SEQ ID NO:
• 9, SEQ ID NO:
• 10, and SEQ ID NO:
• 11;
• ii) any polypeptide that is at least 90% homologous to the polypeptide of i);
• iii) a polypeptide that is encoded by a DNA comprising the sequence of SEQ ID NO:
• 3 or SEQ ID NO:
• 4;
• and
• iv) any polypeptide that is encoded by a polynucleotide that is at least 90% homologous to the polynucleotide of iii;
• wherein said immunogenic composition provides a protective effect against clinical symptoms associated with PCV2 infection after administration of a single dose thereof.