Incoherent lensfree cell holography and microscopy on a chip
AssigneeThe Regents of the University of California
InventorsAydogan Ozcan, Serhan Omer Isikman, Chetin Oztoprak
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Abstract
A system for imaging a cytological sample includes a sample holder configured to hold a cytological sample. A spatial filter is disposed at a distance z1 from the sample holder on first side of the sample holder, the spatial filter having an aperture disposed therein configured to allow the passage of illumination. An imaging sensor array is disposed at a distance z2 from the sample holder on a second, opposite side of the sample holder. An illumination source is configured to illuminate the cytological sample through the aperture, the spatial filter being interposed between the illumination source and the sample holder.
Claims15
A system for imaging a cytological sample comprising:
- a sample holder configured to hold a cytological sample;
- a spatial filter disposed at a distance z1 from the sample holder on a first side of the sample holder, the spatial filter having an aperture disposed therein configured to allow the passage of illumination;
- an image sensor array disposed at a distance z2 from the sample holder on a second, opposite side of the sample holder, wherein z1<<z2;
- an illumination source configured to illuminate the cytological sample through the aperture, the spatial filter being interposed between the illumination source and the sample holder;
- and
- at least one processor configured to receive image frames from the imaging sensor array containing raw holographic images of objects of interest in the cytological sample and further configured to recover a lost hologram phase of the objects of interest and reconstruct a complex image of the objects of interest.
A method of imaging a cytological sample comprising:
- illuminating a front side of a sample holder configured to hold a cytological sample with an illumination source emitting at least partially incoherent light, the at least partially incoherent light passing through an aperture prior to illuminating the cytological sample, wherein the aperture is disposed at a distance z1 from the sample holder and the imaging sensor array is disposed at a distance z2 from an opposing side of the sample holder wherein z2<<z1;
- obtaining one or more image frames from an imaging sensor array disposed on a back side of the sample holder, the one or more image frames containing raw holographic images of objects of interest within the cytological sample;
- processing the one or more image frames containing raw holographic images of objects of interest with a processor to recover lost hologram phase information of the objects of interest contained within the image frames;
- outputting from the processor a reconstructed image of the objects of interest based at least in part on the raw holographic images and the lost hologram phase information.
Dependencies are inferred from the claim text.