# US 9,006,522 B1 · Canola variety inbred NS6454

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- Number: US 9,006,522 B1
- Document ID: US9006522B1
- Status: Granted
- Kind: B1
- Application number: 13/746,981
- Filed: 2013-01-22
- Granted: 2015-04-14
- Earliest claimed: 2013-01-22
- Assignees: Pioneer Hi-Bred International, Inc.
- Applicants: Pioneer Hi-Bred International Inc.
- Inventors: Jayantilal Devabhai Patel; Daniel Joseph Stanton; Ferdinand G. Thoonen
- Web page: https://patentgrep.com/patent/US9006522B1

## CPC

- A01H 6/202: {Brassica napus [canola]}
- A01H 5/10: Seeds
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES

## Abstract

A novel canola variety designated NS6454 and seed, plants and plant parts thereof. Methods for producing a canola plant that comprise crossing canola variety NS6454 with another canola plant. Methods for producing a canola plant containing in its genetic material one or more traits introgressed into NS6454 through backcross conversion and/or transformation, and to the canola seed, plant and plant part produced thereby. Hybrid canola seed, plant or plant part produced by crossing the canola variety NS6454 or a locus conversion of NS6454 with another canola variety.

## Claims

### Claim 1

Canola variety NS6454, representative seed of said variety having been deposited under ATCC accession number PTA-121850.

### Claim 2 (depends on 1)

A seed of the canola variety of claim 1.

### Claim 3 (depends on 2)

The seed of claim 2, further comprising a transgenic event.

### Claim 4 (depends on 3)

The seed of claim 3, wherein the transgenic event confers a trait selected from the group consisting of male sterility, site-specific recombination, abiotic stress tolerance, altered phosphorus, altered antioxidants, altered fatty acids, altered essential amino acids, altered carbohydrates, herbicide resistance, insect resistance and disease resistance.

### Claim 5

Seed of canola variety NS6454, representative seed having been deposited under ATCC accession number PTA-121850, wherein the seed further comprises a locus conversion, and wherein the seed produces a plant otherwise having the same phenotypic traits as NS6454 when grown under the same environmental conditions.

### Claim 6 (depends on 5)

The seed of claim 5, wherein the locus conversion confers a trait selected from the group consisting of male sterility, site-specific recombination, abiotic stress tolerance, altered phosphorus, altered antioxidants, altered fatty acids, altered essential amino acids, altered carbohydrates, herbicide resistance, insect resistance and disease resistance.

### Claim 7 (depends on 1)

A plant or plant part of the canola variety of claim 1.

### Claim 8 (depends on 7)

A canola seed produced by crossing the plant or plant part of claim 7 with a different canola plant.

### Claim 9 (depends on 8)

A canola plant produced by growing the canola seed of claim 8.

### Claim 10 (depends on 9)

A method for producing a second canola plant comprising applying plant breeding techniques to a first canola plant, or parts thereof, wherein said first canola plant is the canola plant of claim 9, and wherein application of said techniques results in the production of said second canola plant.

### Claim 11 (depends on 10)

The method of claim 10, further defined as producing an inbred canola plant derived from the canola line NS6454, the method comprising the steps of:
(a) crossing said first canola plant with itself or another canola plant to produce seed of a subsequent generation;
(b) harvesting and planting the seed of the subsequent generation to produce at least one plant of the subsequent generation;
(c) repeating steps (a) and (b) for an additional 2-10 generations to produce an inbred canola plant derived from canola line NS6454.

### Claim 12 (depends on 10)

The method of claim 10, further defined as producing an inbred canola plant derived from canola line NS6454, the method comprising the steps of:
(a) crossing said first canola plant with an inducer variety to produce haploid seed; and
(b) doubling the haploid seed to produce an inbred canola plant derived from canola line NS6454.

### Claim 13 (depends on 7)

The plant or plant part of claim 7, further comprising a transgenic event.

### Claim 14 (depends on 13)

The plant or plant part of claim 13, wherein the transgenic event confers a trait selected from the group consisting of selected from the group consisting of male sterility, site-specific recombination, abiotic stress tolerance, altered phosphorus, altered antioxidants, altered fatty acids, altered essential amino acids, altered carbohydrates, herbicide resistance, insect resistance and disease resistance.

### Claim 15 (depends on 13)

A canola seed produced by crossing the plant or plant part of claim 13 with a different canola plant.

### Claim 16 (depends on 15)

A canola plant produced by growing the seed of claim 15.

### Claim 17 (depends on 7)

The plant or plant part of claim 7, further comprising a locus conversion.

### Claim 18 (depends on 17)

The plant or plant part of claim 17, wherein the locus conversion confers a trait selected from the group consisting of male sterility, site-specific recombination, abiotic stress tolerance, altered phosphorus, altered antioxidants, altered fatty acids, altered essential amino acids, altered carbohydrates, herbicide resistance, insect resistance and disease resistance.

### Claim 19 (depends on 17)

A canola seed produced by crossing the plant or plant part of claim 17 with a different canola plant.

### Claim 20 (depends on 19)

A canola plant produced by growing the seed of claim 19.

### Claim 21

A seed, plant, plant part or cell of canola variety NS6454, representative seed of said variety having been deposited under ATCC accession number PTA-121850.

### Claim 22 (depends on 21)

The method of crossing the plant or plant part of claim 21 with another plant or plant part to produce F1 seed.

### Claim 23 (depends on 22)

The F1 seed of claim 22.

### Claim 24 (depends on 23)

The method of growing the seed of claim 23 to produce a first canola plant, and crossing the first canola plant with itself or another canola plant.

### Claim 25 (depends on 21)

The seed, plant, plant part or cell of claim 21 further comprising a transgenic event.

### Claim 26 (depends on 25)

The seed of claim 25, wherein the transgenic event confers a trait selected from the group consisting of male sterility, site-specific recombination, abiotic stress tolerance, altered phosphorus, altered antioxidants, altered fatty acids, altered essential amino acids, altered carbohydrates, herbicide resistance, insect resistance and disease resistance.

### Claim 27 (depends on 21)

The seed, plant, plant part or cell of claim 21, further comprising a cytoplasmic conversion.

### Claim 28 (depends on 27)

The seed, plant, plant part or cell of claim 27, wherein the cytoplasmic conversion confers the trait of male sterility.

### Claim 29 (depends on 28)

A method of crossing the plant or plant part of claim 28 with another plant to produce F1 seed.

### Claim 30 (depends on 29)

The F1 seed of claim 29.

### Claim 31 (depends on 30)

A method of growing the seed of claim 30 to produce a first canola plant, and crossing the first canola plant with itself or another canola plant.

### Claim 32

A seed, plant, plant part or cell of canola variety NS6454, representative seed of said variety having been deposited under ATCC accession number PTA-121850 further comprising a locus conversion.

### Claim 33 (depends on 32)

The seed of claim 32, wherein the locus conversion confers a trait selected from the group consisting of male sterility, site-specific recombination, abiotic stress tolerance, altered phosphorus, altered antioxidants, altered fatty acids, altered essential amino acids, altered carbohydrates, herbicide resistance, insect resistance and disease resistance.