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AssigneesMicrobiopharm Japan Co., Ltd., National Institute of Advanced Industrial Science and Technology
InventorsYoshikazu Fujii, Tadashi Fujii, Akira Arisawa, Tomohiro Tamura
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An expression vector capable of expressing a foreign gene in Pseudonocardia autotrophica; a transformant of Pseudonocardia autotrophica produced by using the expression vector; a method for producing a protein by using the transformant; a method for producing an active form of vitamin D3 from vitamin D3, which comprises highly expressing a gene encoding an enzyme involved in the synthesis of the active form of vitamin D3 in a transformant by using the expression vector or the transformant; a method for producing 25-hydroxyvitamin D2 from vitamin D2; and a method for producing pravastatin from compactin, which comprises highly expressing a compactin hydroxylase gene in a transformant by using the expression vector or the transformant.
An expression vector, including a replication initiation region derived from Pseudonocardia autotrophica, a multicloning site for introducing an exogenous gene, an exogenous gene introduced into the multicloning site, a promoter, a terminator, and a defective selection marker, which autonomously replicates in cells of Pseudonocardia autotrophica to enable expression of the exogenous gene introduced, wherein the replication initiation region comprises a base sequence represented by SEQ ID NO:
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