Methods for isolating bacteria
AssigneesDeinove, Centre National De La Recherche Scientifique
InventorsJean-Paul Leonetti, Stéphanie Texier
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Abstract
The present invention relates to compositions and methods to identify novel bacteria and metabolites derived therefrom. More specifically, the invention describes a novel method to isolate bacteria producing metabolites of interest from environmental samples. Particularly, the invention discloses a method to select rare antibiotic producing bacteria. The invention can be used from any sample and allows the isolation of bacteria having e.g., pharmaceutical or agrochemical interest.
Claims20
A method to identify or isolate from a sample a bacterium producing a pharmaceutical compound, the method comprising:
- a) providing a sample comprising uncharacterized and uncultivated bacteria;
- b) subjecting the sample to a repeated sequential irradiation treatment that is sufficient to reduce by at least 2 log the bacterial titer in a culture of E. coli, said treatment comprising at least 2 sequential irradiations at intervals of between 1 and 8 hours;
- c) identifying or isolating a living or growing bacterium having the ability to either reassemble its genome or to resist DNA damage, from said treated sample;
- and
- d) selecting a bacterium of step e) which produces the pharmaceutical compound.
Dependencies are inferred from the claim text.