Cells and methods for producing rhamnolipids

A genetically modified Pseudomonas putida cell, which is able to form at least one rhamnolipid of general formula (I),

• wherein
• m=2, 1 or 0,
• n=1 or 0,
• R1 and R2 are organic residues having 2 to 24 carbon atoms,
• wherein said cell has been genetically modified such that, compared to its wild-type, the cell has an increased activity of at least one of the enzymes E1, E2 and E3, wherein the enzyme E1 has at least 95% amino acid identity to SEQ ID NO:
• 2, the enzyme E2 has at least 95% amino acid identity to SEQ ID NO:
• 4, and the enzyme E3 has at least 95% amino acid identity to SEQ ID NO:
• 6;
• and wherein said cell is also genetically modified such that, compared to its wild-type, the cell has increased activity of at least one enzyme selected from the group consisting of:
• E4, which has at least 95% amino acid identity to SEQ ID NO:
• 10, E5, which has at least 95% amino acid identity to SEQ ID NO:
• 12, E6, which has at least 95% amino acid identity to SEQ ID NO:
• 16, and E7, which has at least 95% amino acid identity to SEQ ID NO:
• 14.