# US 9,005,922 B2 · Sensor chip for screening topoisomerase inhibitor and screening method thereof

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- Number: US 9,005,922 B2
- Document ID: US9005922B2
- Status: Granted
- Kind: B2
- Application number: 12/924,118
- Filed: 2010-09-21
- Published: 2011-11-24
- Granted: 2015-04-14
- Earliest claimed: 2010-05-24
- Assignees: Taipei Medical University
- Applicants: Chun-Mao Lin; Hsiang-Ping Tsai; Chwen-Ming Shih; Jau-Lang Hwang; Chi-Ming Lee
- Inventors: Chun-Mao Lin; Hsiang-Ping Tsai; Chwen-Ming Shih; Jau-Lang Hwang; Chi-Ming Lee
- Web page: https://patentgrep.com/patent/US9005922B2

## CPC

- G01N 33/573: for enzymes or isoenzymes
- C12Q 1/533: involving isomerase
- G01N 2500/00: Screening for compounds of potential therapeutic value
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

The present invention discloses a sensor chip for screening a topoisomerase inhibitor and a screening method thereof. The sensor chip comprises topoisomerase I and a biochip. The topoisomerase I is immobilized on the biochip, and the topoisomerase I has DNA catalytic activity. This provides a rapid screening method for topoisomerase I inhibitors.

## Claims

### Claim 1

A screening method for a topoisomerase inhibitor, comprising the following steps:
immobilizing a topoisomerase, on a micro-fluid chip, by flowing through a solution comprising the topoisomerase with a flow rate of 10-40 μL/min for 60-300 seconds, wherein a pH value of the solution ranges from 3.0-10.0;
adding deoxyribonucleic acid and an inhibitor to be analyzed onto the micro-fluid chip comprising the immobilized topoisomerase; and
detecting a binding activity of the inhibitor to be analyzed and the immobilized topoisomerase by a surface plasmon resonance assay.

### Claim 2 (depends on 1)

The screening method as claimed in claim 1, wherein the deoxyribonucleic acid and the topoisomerase form a topoisomerase-deoxyribonucleic acid cleavage complex.

### Claim 3 (depends on 2)

The screening method as claimed in claim 2, further comprising a step of determining the binding activity of the inhibitor to be analyzed and the topoisomerase-deoxyribonucleic acid cleavage complex by the surface plasmon resonance assay.

### Claim 4 (depends on 1)

The screening method as claimed in claim 1, wherein the binding activity is represented by a variation of resonance units obtained from the surface plasmon resonance assay.

### Claim 5 (depends on 1)

The screening method as claimed in claim 1, wherein the topoisomerase comprises topoisomerase I.

### Claim 6 (depends on 5)

The screening method as claimed in claim 5, wherein the topoisomerase further comprises human topoisomerase I.

### Claim 7 (depends on 5)

The screening method as claimed in claim 5, wherein the inhibitor comprises a camptothecin and derivatives thereof.