# US 9,005,657 B2 · In situ mixing and application of hydrocolloid systems for pre- and post harvest use on agricultural crops > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 9,005,657 B2 - Document ID: US9005657B2 - Status: Granted - Kind: B2 - Application number: 14/448,237 - Filed: 2014-07-31 - Published: 2014-11-20 - Granted: 2015-04-14 - Earliest claimed: 2013-05-05 - Applicants: Nazir Mir - Inventors: Nazir Mir - Web page: https://patentgrep.com/patent/US9005657B2 ## CPC - A01N 27/00: Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons - A01N 37/44: containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids - A01N 43/12: condensed with a carbocyclic ring - A01N 43/32: six-membered rings - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS ## Abstract In-situ methods of applying ethylene response manipulation formulations are disclosed. The formulations comprise at least one ethylene response manipulation agent which is at least partially encapsulated, a polyol liquid medium, or a hydrogel medium, or a combination of polyol and hydrogel medium. A preferred ethylene response manipulation agent is 1-methylcyclopropene. ## Claims ### Claim 1 An in-situ method of reducing loss of 1-methylcyclopropene (1-MCP) in a modifying plant ethylene response formulation to be applied to at least one target plant in a direct soil application comprising the steps of: a) mixing or suspending 1-methylcyclopropene (1-MCP) at least partially encapsulated with at least one encapsulant, in a water based medium, to form a 1-MCP control release mixture comprising encapsulated 1-MCP and free 1-MCP, b) forming a 1-MCP control release liquid formulation from the mixture in a continuous stirred tank system, and c) immediately spraying the 1-MCP control release formulation, within or less than 1 hour of formulation formation, onto at the least one target plant in a direct soil application, wherein less than 80% by weight gaseous or volatile 1-MCP is released into a headspace of an airtight chamber from the 1-MCP controlled release liquid formulation. ### Claim 2 (depends on 1) The method of claim 1 wherein the formulation comprises from about 0.01 to about 10,000 milligrams by weight of said 1-MCP per liter. ### Claim 3 (depends on 1) The method of claim 1 wherein the formulation is applied at a rate of from about 0.1 gram to about 100 grams of said 1-MCP per hour. ### Claim 4 (depends on 1) The method of claim 1 wherein the encapsulant is cyclodextrin. ### Claim 5 (depends on 1) The method of claim 1 wherein the encapsulant is α-cyclodextrin or modified α-cyclodextrin. ### Claim 6 (depends on 1) The method of claim 1 wherein the encapsulant is β-cyclodextrin or modified β-cyclodextrin. ### Claim 7 (depends on 1) The method of claim 1 wherein the encapsulant is γ-cyclodextrin or modified γ-cyclodextrin. ### Claim 8 (depends on 1) The method of claim 1 wherein a ratio of encapsulated to non-encapsulated 1-MCP can be about 99:1 to about 50:50. ### Claim 9 (depends on 1) The method of claim 1 wherein the formulation further comprises at least one pesticide. ### Claim 10 (depends on 9) The method of claim 9 wherein the pesticide is selected from the group consisting of fungicide, insecticide and biopesticide. ### Claim 11 (depends on 1) The method of claim 1 wherein the formulation further comprises at least one plant growth regulator. ### Claim 12 (depends on 11) The method of claim 11 wherein the plant growth regulator is selected from the group consisting of gibberellic acid, etheral and aminoethoxyvinylglycine. ### Claim 13 (depends on 1) The method of claim 1 wherein the formulation further comprises a surfactant. ### Claim 14 (depends on 13) The method of claim 13 wherein the surfactant is selected from the group consisting of anionic, cationic, nonionic, zwitterionic and ampholytic surfactants. ### Claim 15 An in-situ method of reducing loss of 1-methylcyclopropene (1-MCP) in a modifying plant ethylene response formulation to be applied to at least one target plant in a direct soil application using an automated combination mixing and spraying equipment comprising the steps of: a) mixing or suspending 1-methylcyclopropene (1-MCP), at least partially encapsulated with at least one encapsulant, in a water based medium, to form a 1-MCP control release mixture comprising encapsulated 1-MCP and free 1-MCP, b) passing the mixture into a stirred tank system to form a 1-MCP controlled release liquid formulation, and c) immediately spraying the volatile 1-MCP control release liquid formulation onto at least one target plant in a direct soil application within or less than 1 hour of formulation formation such that the formulation remains sprayable and wherein less than 80% by weight gaseous or volatile 1-MCP is released into a headspace of an airtight chamber from the 1-MCP controlled release liquid formulation. ### Claim 16 (depends on 15) The method of claim 15 wherein the encapsulant is cyclodextrin. ### Claim 17 (depends on 15) The method of claim 15 wherein the encapsulant is α-cyclodextrin or modified α-cyclodextrin. ### Claim 18 (depends on 15) The method of claim 15 wherein the encapsulant is β-cyclodextrin or modified β-cyclodextrin. ### Claim 19 (depends on 15) The method of claim 15 wherein the encapsulant is γ-cyclodextrin or modified γ-cyclodextrin. ### Claim 20 (depends on 15) The method of claim 15 wherein the formulation further comprises a surfactant. ### Claim 21 (depends on 20) The method of claim 20 wherein the surfactant is selected from the group consisting of anionic, cationic, nonionic, zwitterionic and ampholytic surfactants. ### Claim 22 An in-situ method of reducing loss of 1-methylcyclopropene (1-MCP) in a modifying plant ethylene response formulation to be applied to at least one target plant in a direct soil application comprising the steps of: a) mixing or suspending 1-methylcyclopropene (1-MCP) powder encapsulated with at least one encapsulant, in a water based medium, to form a volatile 1-MCP control release liquid formulation comprising encapsulated 1-MCP and free 1-MCP, and b) spraying the volatile 1-MCP control release liquid formulation onto at least one target plant in a direct soil application within or less than 1 hour of formulation formation such that the formulation remains sprayable and wherein less than 80% by weight gaseous or volatile 1-MCP is released into a headspace of an airtight chamber from the 1-MCP controlled release liquid formulation. ### Claim 23 (depends on 22) The method of claim 22 wherein the formulation further comprises at least one antimicrobial. ### Claim 24 (depends on 23) The method of claim 23 wherein the antimicrobial is selected from the group consisting of chlorine dioxide, sulphur dioxide, thymol, carvacrol, cinnamaldehyde, allyl isothiocyanate, ethanol, oregano extracts, other synthetic or natural occurring flavanols, phenolic compounds and organic acids. ### Claim 25 (depends on 22) The method of claim 22 wherein the formulation further comprises at least one antioxidant. ### Claim 26 (depends on 25) The method of claim 25 wherein the antioxidant is diphenyl amine or ethoxyquin. ### Claim 27 (depends on 22) The method of claim 22 wherein the formulation further comprises at least one plant growth regulator. ### Claim 28 (depends on 27) The method of claim 27 wherein the plant growth regulator is selected from the group consisting of gibberellic acid, etheral and aminoethoxyvinylglycine. ### Claim 29 (depends on 22) The method of claim 22 wherein the formulation further comprises a surfactant. ### Claim 30 (depends on 29) The method of claim 29 wherein the surfactant is selected from the group consisting of anionic, cationic, nonionic, zwitterionic and ampholytic surfactants.