# US 9,005,543 B2 · Apparatus for punching and solid phase extraction of dried biological fluid spot and related methods

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- Number: US 9,005,543 B2
- Document ID: US9005543B2
- Status: Granted
- Kind: B2
- Application number: 12/917,138
- Filed: 2010-11-01
- Published: 2012-05-03
- Granted: 2015-04-14
- Earliest claimed: 2010-11-01
- Assignees: Agilent Technologies, Inc.
- Applicants: Robert Lee Grenz; William C. Hudson
- Inventors: Robert Lee Grenz; William C. Hudson
- Web page: https://patentgrep.com/patent/US9005543B2

## CPC

- G01N 1/286: {involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising (microtomes G01N1/06; pulverising in general B02C; mixing in general B01F)}
- B01L 3/0275: {Interchangeable or disposable dispensing tips}
- B01L 3/5023: {with a sample being transported to, and subsequently stored in an absorbent for analysis}
- B01L 2300/0672: Integrated piercing tool
- B01L 2300/0681: Filter
- G01N 1/405: {by adsorption or absorption}
- G01N 2001/288: {Filter punches}
- Y10T 436/255: including use of a solid sorbent, semipermeable membrane, or liquid extraction
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

## Abstract

An apparatus for punching and extracting analytes from a dried biological fluid spot includes a tube and a sorbent bed. The tube includes a proximal tube opening, a distal tube opening, and a distal section. The distal section includes a tube wall having a tapered diameter that reduces down to the distal tube opening. The apparatus may also include a tube extension that includes an extension wall having a tapered diameter that reduces from a proximal extension opening to a distal extension opening. The tube extension is movable between an attached position and a detached position. At the attached position, the tube extension is secured to the tube by frictional contact between the extension wall and the tube at the distal tube opening. At the detached position the tube extension is physically separate from the tube. The sorbent bed is disposed at an axial distance from the distal tube opening and in the tube or the tube extension. The sorbent bed has a composition configured for solid phase extraction.

## Claims

### Claim 1

An apparatus for punching and extracting analytes from a dried biological fluid spot, the apparatus comprising:
a tube comprising a proximal tube end circumscribing a proximal tube opening, a distal tube end circumscribing a distal tube opening having an inside diameter less than an inside diameter of the proximal tube opening, and a distal section composed of an inert organic polymer having a Rockwell hardness of 89 or greater, the distal section comprising a distal tube wall having a tapered inside diameter that reduces in a direction from the proximal tube end to the distal tube end, the distal tube wall comprising a first section and a second section adjoining the first section and extending to the distal tube end, the first section having a first section thickness and the second section having a second section thickness tapering from the first section thickness to a thickness of the distal tube end less than the first section thickness, wherein the distal tube end comprises a sharp edge; and hardness effective for punching through a substrate on which the dried biological fluid spot is formed such that a sample unit comprising the dried biological fluid spot and a punched portion of the substrate passes through the distal tube opening and is captured in the distal section; and
a sorbent disposed in the distal section at a distance from the distal tube opening such that the sorbent bed prevents passage of the sample unit to the proximal tube opening, the sorbent bed having a composition configured for solid phase extraction.

### Claim 2 (depends on 1)

The apparatus of claim 1, wherein the first section thickness is 0.5 mm or greater and the second section thickness is less than 0.5 mm at the distal tube end.

### Claim 3 (depends on 1)

The apparatus of claim 1, wherein the composition of the sorbent bed is selected from the group consisting of an organic polymer, an organic copolymer, an organic polymer blend, a glass fiber cellulose, a metal oxide or metalloid oxide, an ion exchange modified metal oxide or metalloid oxide, a functionalized metal oxide or metalloid oxide, and combinations of two or more of the foregoing.

### Claim 4 (depends on 1)

The apparatus of claim 1, wherein the composition of the sorbent bed is configured for retaining analytes from a dried biological fluid spot, or for retaining non-analytical material while passing analytes.

### Claim 5 (depends on 1)

The apparatus of claim 1, wherein the distal section comprises an inner shoulder and the sorbent bed is disposed on the inner shoulder.

### Claim 6 (depends on 1)

The apparatus of claim 1, comprising a tube extension comprising a proximal extension end circumscribing a proximal extension opening, a distal extension end circumscribing a distal extension opening, and an extension wall having a tapered inside diameter that reduces from the proximal extension opening to the distal extension opening, wherein the proximal extension opening has an inside diameter greater than an outside diameter of the distal end, the distal extension opening has an inside diameter less than the inside diameter of the distal tube opening, and the tube extension is movable between an attached position and a detached position, and wherein:
at the attached position, the tube extension is secured to the tube by frictional contact between the extension wall and the distal tube end, and the distal extension opening fluidly communicates with the distal tube opening; and
at the detached position the tube extension is physically separate from the tube.

### Claim 7 (depends on 1)

The apparatus of claim 1, wherein the tube has a composition selected from the group consisting of polyolefins, polypropylene, polyethylene, polyamide, polyacrylate, a combination of two of more of the foregoing.

### Claim 8

An apparatus for punching and extracting analytes from a dried biological fluid spot, the apparatus comprising:
a tube comprising a proximal tube end circumscribing a proximal tube opening, a distal tube end circumscribing a distal tube opening having an inside diameter less than an inside diameter of the proximal tube opening, and a distal section terminating at the distal tube end, the distal section comprising a distal tube wall having a tapered inside diameter that reduces in a direction from the proximal tube end to the distal tube end, the distal tube wall comprising a first section and a second section adjoining the first section and extending to the distal tube end, the first section having a first section thickness and the second section having a second section thickness tapering from the first section thickness to a thickness of the distal tube end less than the first section thickness, wherein the distal tube end comprises a sharp edge and hardness effective for punching through a substrate on which the dried biological fluid spot is formed such that a sample unit comprising the dried biological fluid spot and a punched portion of the substrate passes though the distal tube opening and is captured in the distal section;
a frit disposed in the distal section at a distance from the distal tube opening such that the frit prevents passage of the sample unit to the proximal tube opening;
a tube extension comprising a proximal extension end circumscribing a proximal extension opening, a distal extension end circumscribing a distal extension opening, and an extension wall having a tapered inside diameter that reduces from the proximal extension opening to the distal extension opening, wherein the proximal extension opening has an inside diameter greater than an outside diameter of the distal tube end, the distal extension opening has an inside diameter less than the inside diameter of the distal tube opening, and the tube extension is movable between an attached position and a detached position, wherein:
at the attached position, the tube extension is secured to the tube by frictional contact between the extension wall and the distal tube end, and the distal extension opening fluidly communicates with the distal tube opening; and
at the detached position the tube extension is physically separate from the tube; and
a sorbent bed disposed in the tube extension, the sorbent bed having a composition configured for solid phase extraction.

### Claim 9 (depends on 8)

The apparatus of claim 8, wherein the frit is fixed in position by frictional contact with the inside tube wall.