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US 9,002,077 B2Granted
Visualization of stained samples
AssigneeCambridge Research & Instrumentation, Inc.
InventorsClifford C. Hoyt, Richard Levenson
Life of this patent
Estimating the term…
Abstract
Methods and systems are disclosed that include: applying an immunohistochemical stain, eosin, and a counterstain to a sample; obtaining a plurality of images of the sample, each of the plurality of images corresponding to radiation from the sample in a different wavelength band; decomposing the plurality of images of the sample to obtain component images corresponding to the immunohistochemical stain, eosin, and the counterstain; and generating a sample image based on the component images, where the sample image includes contributions from the counterstain and from one of the immunohistochemical stain and eosin, and substantially not from the other of the immunohistochemical stain and eosin.
Claims31
A method, comprising:
- applying an immunohistochemical stain, eosin, and a counterstain to a sample;
- obtaining a plurality of images of the sample, each of the plurality of images corresponding to radiation from the sample in a different wavelength band;
- decomposing the plurality of images of the sample to obtain component images corresponding to the immunohistochemical stain, eosin, and the counterstain;
- and
- generating first and second sample images based on the component images derived from the plurality of images,
- wherein the first sample image comprises contributions from the component images corresponding to the counterstain and to the immunohistochemical stain, and substantially not from the component image corresponding to eosin;
- wherein the second sample image comprises contributions from the component images corresponding to the counterstain and to eosin, and substantially not from the component image corresponding to the immunohistochemical stain;
- and
- wherein each of the component images that contributes to the first and second sample images corresponds to absorption of radiation by a component of the sample.
6 more elements
A method, comprising:
- applying an immunohistochemical stain, eosin, and a counterstain to a sample;
- obtaining a plurality of images of the sample, each of the plurality of images corresponding to radiation from the sample in a different wavelength band;
- and
- using an electronic processor to:
- (i) decompose the plurality of images of the sample to obtain component images corresponding to the immunohistochemical stain, eosin, and the counterstain;
- (ii) analyze one or more of the component images to identify one or more regions of interest in the sample;
- (iii) determine an amount of the immunohistochemical stain in one or more of the regions of interest based on at least some of the component images, wherein each of the at least some of the component images corresponds to absorption of radiation by a component of the sample;
- and
- (iv) output one or more indicators corresponding to the determined amount of the immunohistochemical stain.
6 more elements
A method, comprising:
- applying an immunohistochemical stain, eosin, and a counterstain to a sample;
- obtaining a plurality of images of the sample, each of the plurality of images corresponding to radiation from the sample in a different wavelength band;
- decomposing the plurality of images of the sample to obtain component images corresponding to the immunohistochemical stain, eosin, and the counterstain;
- and
- generating a sample image based on the eosin and counterstain component images,
- wherein the sample image comprises an indicator based on information derived from the immunohistochemical component image;
- and
- wherein each of the component images that contributes to the sample image corresponds to absorption of radiation by a component of the sample.
5 more elements
A system, comprising:
- a source configured to direct radiation to a sample comprising an immunohistochemical stain, eosin, and a counterstain;
- a detector configured to measure radiation emitted from the sample to obtain a plurality of images of the sample, wherein each of the plurality of images corresponds to radiation from the sample in a different wavelength band;
- and
- an electronic processor configured to:
- (i) decompose the plurality of images of the sample to obtain component images corresponding to the immunohistochemical stain, eosin, and the counterstain;
- and
- (ii) generate first and second sample images based on the component images derived from the plurality of images,
- wherein the first sample image comprises contributions from the component images corresponding to the counterstain and to the immunohistochemical stain, and substantially not from the component image corresponding to eosin;
- wherein the second sample image comprises contributions from the component images corresponding to the counterstain and to eosin, and substantially not from the component image corresponding to the immunohistochemical stain;
- and
- wherein each of the component images that contributes to the first and second sample images corresponds to absorption of radiation by a component of the sample.
8 more elements
A system, comprising:
- a source configured to direct radiation to a sample comprising an immunohistochemical stain, eosin, and a counterstain;
- a detector configured to measure radiation emitted from the sample to obtain a plurality of images of the sample, wherein each of the plurality of images corresponds to radiation from the sample in a different wavelength band;
- and
- an electronic processor configured to:
- (i) decompose the plurality of images of the sample to obtain component images corresponding to the immunohistochemical stain, eosin, and the counterstain;
- (ii) analyze one or more of the component images to identify one or more regions of interest in the sample;
- (iii) determine an amount of the immunohistochemical stain in one or more of the regions of interest based on at least some of the component images, wherein each of the at least some of the component images corresponds to absorption of radiation by a component of the sample;
- and
- (iv) output one or more indicators corresponding to the determined amount of the immunohistochemical stain.
6 more elements
Dependencies are inferred from the claim text.