Compositions and methods for assaying markers tightly linked to resistance locus Bs2 of pepper
AssigneeMonsanto Technology LLC
InventorsCarl Martin Jones, Diane Kerr Avner
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Abstract
The invention relates to compositions and methods for genotypically screening pepper lines for the presence of a polymorphism at the Bs2_5859 locus genetically linked to the Bs2 gene conferring resistance to Bacterial Spot caused by Xanthomonas campestris. Further provided are methods for producing plants displaying Bs2-mediated resistance, and fruit and seeds therefrom.
Claims31
A method for determining the genotype of a pepper plant for Bacterial Spot resistance, comprising obtaining a sample of nucleic acids from said plant and detecting in said nucleic acids a polymorphism at the Bs2—5859 locus that is genetically linked to Bacterial Spot Resistance.
A method of producing a pepper plant having Bs2-mediated resistance to Bacterial Spot comprising the steps of:
- (a) crossing a pepper plant having Bs2-mediated resistance with a second pepper plant;
- (b) assaying at least a first progeny pepper plant for a polymorphism at the Bs2—5859 locus that is genetically linked to resistance to Bacterial Spot;
- and
- (c) selecting the at least first progeny plant comprising the polymorphism genetically linked to resistance to Bacterial Spot.
Dependencies are inferred from the claim text.