Bacillus thuringiensis gene with lepidopteran activity
AssigneePioneer Hi-Bred International, Inc.
InventorsAndre R. Abad, Hua Dong, Sue B. Lo, Xiaomei Shi, Thomas C. Wolfe
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Abstract
The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
Claims13
A nucleic acid molecule comprising a codon optimized sequence that has been designed for expression in a plant selected from the group consisting of:
- (a) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence of SEQ 10 NO:
- 2;
- and
- (b) a nucleotide sequence encoding a protein comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:
- 2.
A DNA construct comprising a nucleic acid molecule selected from the group consisting of:
- (a) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:
- 1 or a full-length complement thereof;
- (b) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 2;
- and
- (c) a nucleotide sequence encoding a protein comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:
- 2,
- and a heterologous promoter operably linked to the nucleic acid molecule.
A plant cell having stably incorporated into its genome a DNA construct comprising a nucleotide sequence that encodes a protein having pesticidal activity, wherein said nucleotide sequence is selected from the group consisting of:
- (a) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:
- 1 or a full-length complement thereof;
- (b) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 2;
- and
- (c) a nucleotide sequence encoding a protein comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:
- 2,
- wherein said nucleotide sequence is operably linked to a promoter that drives expression of a coding sequence in a plant cell.
A method for protecting a plant from a pest, comprising introducing into said plant or cell thereof at least one expression vector comprising a nucleotide sequence that encodes a pesticidal polypeptide, wherein said nucleotide sequence is selected from the group consisting of:
- (a) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:
- 1 or a full-length complement thereof;
- (b) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 2;
- and
- (c) a nucleotide sequence encoding a protein comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.