Localization and characterization of flavivirus envelope glycoprotein cross-reactive epitopes and methods for their use
AssigneeThe United States of America as represented by the Secretary of the Department of Health and Human Services, Centers for Disease Control and Prevention
InventorsGwong-Jen J. Chang, Wayne D. Crill
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Abstract
Disclosed herein is a method for identifying flavivirus cross-reactive epitopes. Also provided are flavivirus E-glycoprotein cross-reactive epitopes and flavivirus E-glycoprotein cross-reactive epitopes having reduced or ablated cross-reactivity (and polypeptides comprising such epitopes), as well as methods of using these molecules to elicit an immune response against a flavivirus and to detect a flaviviral infection.
Claims21
An isolated nucleic acid molecule encoding a mutant flavivirus E-glycoprotein polypeptide comprising at least one amino acid substitution at position 104 or 106 compared to a wild type flavivirus E-glycoprotein polypeptide, wherein the mutant E-glycoprotein polypeptide exhibits reduced antibody cross-reactivity as compared to the wild type E-glycoprotein polypeptide, and wherein the flavivirus is selected from the group consisting of dengue serotype 1 virus, dengue serotype 2 virus, dengue serotype 3 virus, dengue serotype 4 virus, St. Louis encephalitis virus, and West Nile virus.
An isolated nucleic acid molecule encoding a mutant flavivirus E-glycoprotein polypeptide comprising an amino acid substitution at at least two of positions 104, 106, or 107 compared to a wild type flavivirus E-glycoprotein polypeptide, wherein the mutant E-glycoprotein polypeptide exhibits reduced antibody cross-reactivity as compared to the wild type E-glycoprotein polypeptide and wherein the flavivirus is selected from the group consisting of dengue serotype 1 virus, dengue serotype 2 virus, dengue serotype 3 virus, dengue serotype 4 virus, St. Louis encephalitis virus, and West Nile virus.
Dependencies are inferred from the claim text.