Cysteine engineered antibodies and conjugates
AssigneeGenentech, Inc.
InventorsSunil Bhakta, Jagath R. Junutula
Life of this patent
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Abstract
Cysteine engineered antibodies comprising a free cysteine amino acid in the heavy chain or light chain are prepared by mutagenizing a nucleic acid sequence of a parent antibody and replacing one or more amino acid residues by cysteine to encode the cysteine engineered antibody; expressing the cysteine engineered antibody; and isolating the cysteine engineered antibody. Certain highly reactive cysteine engineered antibodies were identified by the PHESELECTOR assay. Isolated cysteine engineered antibodies may be covalently attached to a capture label, a detection label, a drug moiety, or a solid support.
Claims28
An isolated cysteine engineered antibody comprising a free cysteine amino acid and a sequence in the heavy chain selected from
- HC-F27C
- CAASGCNIKDT
- SEQ ID NO:
- 56
- HC-K76C
- SADTSCNTAYL
- SEQ ID NO:
- 66
- HC-T78C
- DTSKNCAYLQM
- SEQ ID NO:
- 67
- HC-L81C
- KNTAYCQMNSL
- SEQ ID NO:
- 69
- or a sequence in the light chain selected from
- LC-I75C
- DFTLTCSSLQP
- SEQ ID NO:
- 106
- LC-Y92C
- YCQQHCTTPPT
- SEQ ID NO:
- 110
- LC-N137C
- VVCLLCNFYPR
- SEQ ID NO:
- 127
- LC-N138C
- VCLLNCFYPRE
- SEQ ID NO:
- 128
- LC-K149C
- AKVQWCVDNAL
- SEQ ID NO:
- 133
- wherein a cysteine in the sequence is the free cysteine amino acid.
Dependencies are inferred from the claim text.