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US 8,999,943 B2Granted
Antigene oligomers inhibit transcription
AssigneeBoard of Regents, The University of Texas System
InventorsDavid Reid Corey, Bethany Ann Janowski
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Abstract
Transcription of a gene in a mammalian cell is methylase-independently inhibited by contacting the cell with a nucleic acid oligomer of 12-28 bases complementary for a partially single-stranded target genomic sequence of the gene.
Claims20
A method of methylase-independently inhibiting transcription of a gene in a mammalian cell, the method comprising the steps of:
- contacting the cell with a nucleic acid oligomer of 12-28 bases complementary to a partially single-stranded target genomic sequence of the gene, but not to an mRNA of the gene, whereby the transcription of the gene is subject to a resultant methylase-independent inhibition;
- and
- detecting the resultant methylase-independent inhibition, wherein the oligomer is a double-stranded RNA, and the target genomic sequence is located between nucleotides −50 to +25 from the gene's transcription start site.
Dependencies are inferred from the claim text.