Process for diagnosing rheumatic diseases
AssigneeCypress Bioscience, Inc.
InventorsHolger Bang, Wigbert Berg
Life of this patent
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Abstract
The invention relates to polypeptides reacting with rheumatism-associated autoantibodies. The invention moreover relates to a diagnostic agent comprising any of said polypeptides, to a diagnostic kit comprising said diagnostic agent and to a process for in vitro detection of rheumatic diseases. The invention furthermore relates to a medicament comprising any of said polypeptides and to the use of said polypeptides for preparing a medicament for the prophylaxis and/or treatment of rheumatic diseases.
Claims39
A polypeptide variant of native vimentin comprising SEQ ID NO:
- 1, wherein said polypeptide variant comprises an amino acid sequence wherein
- at least one amino acid residue at position 16, 17, 19, 41, 58, 59, 60, 68, 76, 140, 142, 147, 363, 406 or 452 in said SEQ ID NO:
- 1 is substituted by arginine and wherein optionally
- (a) at least one amino acid residue at positions 3, 20, 33, 36, 37, 94, 165, 361, 399 or 426 in said SEQ ID NO:
- 1 is substituted by leucine;
- (b) at least one amino acid residue at positions 21, 41, 43, 50, 54, 62, 64 or 89 in said SEQ ID NO:
- 1 is substituted by proline;
- (c) at least one amino acid residue at positions 24, 35 or 99 in said SEQ ID NO:
- 1 is substituted by threonine;
- or
- (d) at least one amino acid residue at positions 25, 39, 42, 49, 55 or 67 in said SEQ ID NO:
- 1 is substituted by tyrosine.
A polypeptide fragment which comprises at least six contiguous amino acids of a polypeptide variant of native vimentin and which exhibits reactivity to rheumatoid arthritis-associated autoantibodies, wherein said native vimentin comprises the amino acid sequence set forth in SEQ ID NO:
- 1 and said variant comprises an amino acid sequence which is located within the region of amino acids 30-70 of said SEQ ID NO:
- 1, wherein at least one amino acid residue at position 41, 58, 59, 60, or 68 in said SEQ ID NO:
- 1 is substituted for arginine, and wherein at least one of said six contiguous amino acids in said polypeptide fragment is said substituted arginine.
A peptide derivative which is a retro polypeptide, an inverso polypeptide or a cyclic polypeptide of a variant of native vimentin comprising SEQ ID NO:
- 1, wherein said variant comprises an amino acid sequence wherein at least one amino acid residue at position 16, 17, 19, 41, 58, 59, 60, 68, 76, 140, 142, 147, 363, 406 or 452 in said SEQ ID NO:
- 1 is substituted by arginine.
A polypeptide variant of native vimentin comprising SEQ ID NO:
- 1, wherein said variant comprises an amino acid sequence wherein glycine at positions 16, 41, 59 and 76 in said SEQ ID NO:
- 1 is substituted for arginine and which exhibits reactivity to rheumatoid arthritis-associated autoantibodies.
A 17-mer fragment of a polypeptide variant of native vimentin comprising SEQ ID NO:
- 1, wherein said polypeptide variant comprises an amino acid sequence wherein
- (a) at least one glycine at amino acid residue at position 16, 17, 19, 41, 58, 59, 76, 140, 142, 147, 363, 406 or 452 in said SEQ ID NO:
- 1 is substituted by arginine;
- or
- (b) at least one valine at amino acid residue at position 60 or 68 in said SEQ ID NO:
- 1 is substituted by arginine;
- or
- (c) at least one glycine at amino acid residue at position 16, 17, 19, 41, 58, 59, 76, 140, 142, 147, 363, 406 or 452 in said SEQ ID NO:
- 1 is substituted by arginine and at least one valine at amino acid residue at position 60 or 68 in said SEQ ID NO:
- 1 is substituted by arginine;
- wherein at least one of the 17 amino acids in said fragment of said polypeptide variant of native vimentin is said substituted arginine.
A polypeptide fragment which comprises at least six contiguous amino acids of a polypeptide variant of native vimentin, which polypeptide variant comprises the amino acid sequence of SEQ ID NO:
- 9 and which further exhibits reactivity to rheumatoid arthritis-associated autoantibodies, wherein at least one of said six contiguous amino acids in said polypeptide fragment is the arginine at position 16, 17, 19, 41, 58, 59, 60, 68, 76, 140, 142, 147, 363, 406 or 452 in said SEQ ID NO:
- 9.
Dependencies are inferred from the claim text.