Alpha-amylase mutants
AssigneeNovozymes A/S
InventorsAllan Svendsen, Torben Vedel Borchert, Henrik Bisgaard-Frantzen
Life of this patent
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Abstract
The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to the parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency and specific activity.
Claims13
A variant of a parent alpha-amylase, which variant has alpha-amylase activity and has at least 95% sequence identity with SEQ ID NO:
- 2, 4, 6, 38, 39 or 40, and comprises one or more substitutions at positions H68, H91, H247, R305, K306, H382, K389, H405, H406, H450 or R483 corresponding to SEQ ID NO:
- 2.
An isolated variant of a parent alpha-amylase, wherein:
- (a) the variant has at least 95% sequence identity to SEQ ID NO:
- 2, 4, 6, 38, 39 or 40,
- (b) the variant comprises a substitution at positions H68, H91, H247, R305, K306, H382, K389, H405, H406, H450 or R483 relative to the parent alpha-amylase, using the amino acid sequence of SEQ ID NO:
- 2 for determining position numbering.
An isolated variant of a parent Bacillus licheniformis alpha-amylase, wherein:
- (a) the variant has at least 95% sequence identity to SEQ ID NO:
- 2,
- (b) the variant comprises a substitution at position H68 relative to the parent alpha-amylase, using the amino acid sequence of SEQ ID NO:
- 2 for determining position numbering.
Dependencies are inferred from the claim text.