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US 8,999,894 B2Granted
Nucleic acid-like proteins
AssigneeAlbert Einstein College of Medicine of Yeshiva University
InventorsJohn S. Blanchard, Matthew W. Vetting, Subray S. Hegde
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Abstract
Methods are provided for labeling and for detecting DNA-interacting proteins with an assayable label, comprising combining the DNA-interacting protein with a mfpA pentapeptide repeat family protein that has been purified and that binds to the protein, wherein the mfpA pentapeptide repeat family protein is bound to an assayable label.
Claims9
A method of detecting a DNA-interacting protein, the method comprising
- (a) contacting a protein with a purified Mycobacterium tuberculosis MfpA pentapeptide repeat protein (MtMfpA) which has had an assayable label bound thereto, wherein the label is a visible label, a fluorescent protein, a fluorescent organic compound less than 2000 molecular weight, a radioactive molecule, or an oligohistidine sequence, under conditions permitting the MtMfpA pentapeptide repeat protein to bind to a DNA-interacting protein, and
- (b) detecting the visible label, fluorescent protein, fluorescent organic compound less than 2000 molecular weight, radioactive molecule, or oligohistidine sequence, respectively, of the labeled MtMfpA that is bound to the DNA-interacting protein, so as to thereby detect the DNA-interacting protein.
Dependencies are inferred from the claim text.