Immunochromatography method

An immunochromatography method, which comprises:

• spreading a test substance, a first labeling substance modified with a first binding substance that binds to the test substance, and a second labeling substance modified with the first binding substance that binds to the test substance, which are in a mixed state, on an insoluble carrier;
• capturing the test substance, the first labeling substance, and/or the second labeling substance at a reaction site on the insoluble carrier comprising an immobilizing reagent capable of binding to the test substance, so as to detect the test substance,
• measuring the second labeling substance at the reaction site on the insoluble carrier;
• and thereafter
• amplifying the first labeling substance at the reaction site on the insoluble carrier;
• and thereafter
• measuring the first labeling substance at the reaction site on the insoluble carrier,
• wherein the first labeling substance is a labeling substance that causes an amplification reaction, and the second labeling substance is a labeling substance that does not substantially influence on the amplification reaction, wherein the first labeling substance is a metal colloid and the second labeling substance is a colored particle, and wherein
• the first labeling substance is a substance that reacts with silver ions and a reducing agent used for such silver ions to generate metallic silver, and the second labeling substance is a substance that does not substantially have a reactivity with silver ions and a reducing agent used for the silver ions, and wherein
• the number of metal colloid particles which are applied to the insoluble carrier is from 1×105 or more to 1×108 or less.