Compositions and methods comprising cellulase variants with reduced affinity to non-cellulosic materials
AssigneeDanisco US Inc.
InventorsLuis G. Cascao-Pereira, Thijs Kaper, Bradley R. Kelemen, Amy D. Liu
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Abstract
The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having reduced binding to non-cellulosic materials. Also described are nucleic acids encoding the cellulase, compositions comprising said cellulase, methods of identifying cellulose variants and methods of using the compositions.
Claims14
A cellulase variant obtained from a parent cellulase, wherein said variant is a mature form having cellulase activity, comprises a sequence that is at least 95% identical to the amino acid sequence of SEQ ID NO:3, and comprises a substitution at position 203, wherein the positions are numbered by correspondence with the amino acid sequence of SEQ ID NO:3, and wherein the substitution causes the cellulase variant to have a more negative net charge in comparison to the parent cellulase.
Dependencies are inferred from the claim text.