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US 8,999,686 B2Granted
Methods and compositions for producing fatty alcohols
AssigneeReg Life Sciences, LLC
InventorsZhihao Hu, Vikranth Arlagadda
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Abstract
Methods and compositions, including nucleotide sequences, amino acid sequences, and host cells, for producing fatty alcohols are described.
Claims31
A method of producing a fatty alcohol composition comprising C12 and C14 fatty alcohols in a bacterial cell, the method comprising:
- (a) expressing in the bacterial cell a gene encoding a polypeptide having thioesterase (EC 3.1.2- or EC 3.1.1.5) activity effective to release a fatty acid from acyl-ACP;
- and
- (b) further expressing in said bacterial cell an exogenous gene encoding a polypeptide having the amino acid sequence of SEQ ID NO:22;
- and
- (c) culturing said bacterial cell in culture media containing a carbohydrate carbon source under conditions wherein at least 25 mg of the composition is produced per liter of bacterial culture.
2 more elements
A method of producing a fatty alcohol composition comprising C12 and C14 fatty alcohols in a bacterial cell, the method comprising:
- culturing the bacterial cell at 37° C. in a culture media containing a carbohydrate carbon source, wherein the bacterial cell comprises polynucleotide sequences encoding:
- (a) a first polypeptide having thioesterase (EC 3.1.2.14 or EC 3.1.1.5) activity effective to release a fatty acid from acyl-ACP, and
- (b) a second polypeptide having carboxylic acid reductase activity and comprising an amino acid sequence
- (i) having at least 95% sequence identity to SEQ ID NO:22, and
- (ii) having activity effective to convert a fatty acid to a fatty aldehyde, wherein the second polypeptide is expressed from an exogenous gene, wherein the second polypeptide comprises SEQ ID NO:7, SEQ ID NO:
- 8, SEQ ID NO:9 and SEQ ID NO:
- 10;
- and wherein the composition is produced at a yield of at least 300 mg/L of bacterial culture.
5 more elements
Dependencies are inferred from the claim text.