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US 8,999,684 B2Granted
Candida tropicalis cells and use thereof
AssigneeEvonik Degussa GmbH
InventorsMarkus Poetter, Hans-Georg Hennemann, Steffen Schaffer, Thomas Haas
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Abstract
The invention relates to genetically engineered Candida tropicalis cells, use thereof and a method of production of ω-hydroxycarboxylic acids and ω-hydroxycarboxylic acid esters.
Claims10
A method for production of ω-aminocarboxylic acids or ω-aminocarboxylic acid esters, comprising
- a) contacting a Candida tropicalis cell with a medium comprising a carboxylic acid or a carboxylic acid ester,
- b) cultivating the Candida tropicalis cell under conditions that enable the Candida tropicalis cell to form corresponding ω-hydroxycarboxylic acid or ω-hydroxycarboxylic acid esters from the carboxylic acid or the carboxylic acid ester,
- c) optionally isolating the ω-hydroxycarboxylic acid or ω-hydroxycarboxylic acid esters that formed, converting the ω-hydroxycarboxylic acids or corresponding esters into the ω-oxo-carboxylic acids or the corresponding esters and then aminating the oxo-group,
- wherein the Candida tropicalis cell has, compared with its wild type, a reduced activity of at least one of enzyme encoded by an intron-free nucleic acid sequences selected from the group consisting of SEQ ID NO:
- 57 and a sequence that is 95% identical to SEQ ID NO:
- 57.
3 more elements
Dependencies are inferred from the claim text.