Detection and visualization of the cell cycle in living cells
AssigneeLudwig-Maximilians-Universitat Munchen
InventorsUlrich Rothbauer, Heinrich Leonhardt, Kourosh Zolghadr, Tina Romer, Katrin Schmidthals
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Abstract
The present invention relates to a nucleic acid molecule encoding a polypeptide specifically binding to proliferating cell nuclear antigen (PCNA). The present invention also relates to a vector comprising the nucleic acid molecule of the invention, a host cell comprising the nucleic acid molecule of or the vector of the invention and a method of detecting the amount and/or location of PCNA in living cells, a method of screening for compounds having an effect on the cell cycle.
Claims16
A synthetic nucleic acid molecule encoding a polypeptide which specifically binds to proliferating cell nuclear antigen (PCNA), said nucleic acid molecule comprising a nucleic acid sequence encoding the amino acid sequence of SEQ ID NO:
- 2 or an amino acid sequence deviating from SEQ ID NO:
- 2 by conservative substitution of 2 or 3 amino acids in position 1 to 28 and 38 to 52 of SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.