Production of canola protein isolate without heat treatment
AssigneeBurcon Nutra Science (MB) Corp.
InventorsKevin I. Segall, Brent E. Green, Martin Schweizer
Life of this patent
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Abstract
The supernatant from the deposition of canola protein micellar mass is processed to provide a canola protein isolate which is soluble in an aqueous acidic environment.
Claims27
A process of preparing a canola protein isolate having a protein content of at least 90 wt % (N×6.25) on a dry weight basis, which comprises:
- adding a calcium salt to supernatant from the precipitation of a canola protein micellar mass to provide a conductivity of about 5 mS to about 30 mS to form a calcium phytate precipitate,
- removing precipitated calcium phytate from the resulting solution to provide a clear solution,
- adjusting the pH of the clear solution to about 2.0 to about 4.0,
- concentrating the pH-adjusted clear solution to a protein content of at least about 50 g/L to produce a clear concentrated canola protein solution,
- optionally diafiltering the clear concentrated canola protein solution,
- optionally effecting a colour removal step, and
- drying the concentrated protein solution.
A process of preparing a canola protein isolate having a protein content of at least 90 wt % (N×6.25) on a dry weight basis, which comprises:
- partially concentrating the supernatant from the precipitation of a canola protein micellar mass to a concentration of about 50 g/L or less
- adding a calcium salt to the partially concentrated supernatant to provide a conductivity of about 2 mS to about 30 mS to precipitate calcium phytate from the partially concentrated supernatant,
- removing the precipitated calcium phytate from the resulting solution to provide a clear solution,
- adjusting the pH of the clear solution to about 2.0 to about 4.0,
- concentrating the pH-adjusted clear solution to a protein content of at least about 50 g/L to produce a clear concentrated canola protein solution,
- optionally diafiltering the clear concentrated canola protein solution,
- optionally effecting a colour removal step, and
- drying the concentrated protein solution.
A process of preparing a canola protein isolate having a protein content of at least 90 wt % (N×6.25) on a dry weight basis, which comprises:
- concentrating supernatant from the precipitation of a canola protein micellar mass to a protein content of at least about 50 g/L to produce a concentrated supernatant,
- adding a calcium salt to the concentrated supernatant to provide a conductivity of about 2 to about 30 mS to cause precipitation of calcium phytate,
- removing calcium phytate from the resulting solution to provide a clear solution,
- adjusting the pH of the clear solution to about 2.0 to about 4.0,
- optionally diafiltering the clear pH-adjusted solution,
- optionally effecting a colour removal step, and
- drying the clear optionally pH-adjusted protein solution.
Dependencies are inferred from the claim text.