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US 8,999,176 B2Granted
Isolation of nucleic acids
AssigneeExact Sciences Corporation
InventorsMichael J. Domanico, James P. Light, II, Keith Kopitzke, John Zeis
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Abstract
Provided herein is technology relating to isolating nucleic acids. In particular, the technology relates to methods and kits for extracting nucleic acids from problematic samples such as stool.
Claims5
A method of producing a filtrate from a sample, the method comprising:
- a) placing a sample to be filtered into a spin filter, said spin filter comprising
- i) a hollow body (1);
- ii) a bottom end (2);
- and
- iii) an opening (3) at a top end of said hollow body (1) opposite the bottom end (2),
- wherein said hollow body (1) and said bottom end (2) are both composed of the same porous filtering material;
- and
- b) centrifuging said spin filter,
- wherein during said centrifuging, a fraction of said sample passes through said porous filtering material of said spin filter to produce a filtrate.
6 more elements
Dependencies are inferred from the claim text.