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US 8,999,157 B2Granted
Method for preparation of a biomolecule adsorbent
AssigneeGE Healthcare Bio-Sciences AB
InventorsLars C. Andersson, Anna Arefalk, Ola Lind, Bjorn Noren
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Abstract
The present invention is relates to method for production of a biomolecule adsorbent with a pentadentate ligand with very strong chelating properties, and to methods for use thereof for, for example, protein purification, detection or binding. The biomolecule adsorbent comprises the formula: wherein Q is a carrier, S is a spacer, L is an amide linkage, X is COOH, and n=2 or 3.
Claims8
A method of preparing a biomolecule adsorbent with a pentadentate ligand of the formula
- wherein
- Q is a carrier
- S is a spacer
- L is an amide linkage
- X is COOH
- n =2 or 3
- comprising the following steps:
- providing an alkylene diamine tetraacetic acid dianhydride;
- providing an amino activated carrier;
- coupling said alkylene diamine tetraacetic acid dianhydride to said carrier to form ligands comprised of alkylene diamine triacetic acid linked to said carrier via an amide linkage and a spacer;
- and
- immobilizing charged metal ion ions by charging the adsorbent so obtained with metal ions, selected from Cu2+, Ni2+, Zn2+, Co2+, Fe3+ and Ga3+ to form pentadentate ligands.
9 more elements
Dependencies are inferred from the claim text.