Utilization of phosphoketolase in the production of mevalonate, isoprenoid precursors, and isoprene
AssigneesDanisco US Inc., The Goodyear Tire & Rubber Company
InventorsZachary Q. Beck, Andrew C. Eliot, Caroline M. Peres, Dmitrii V. Vaviline
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Abstract
The invention provides for methods for the production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in cells via the heterologous expression of phosphoketolase enzymes.
Claims52
Recombinant microbial cells capable of producing isoprene from a carbon source, wherein the cells comprise one or more heterologous nucleic acids encoding a polypeptide having phosphoketolase activity and (i) one or more nucleic acids encoding one or more polypeptides of the complete MVA pathway and (ii) a heterologous nucleic acid encoding a polypeptide having isoprene synthase activity, wherein culturing of said recombinant cells in a suitable media provides for the production of isoprene, wherein the production of isoprene occurs without an increase in intracellular acetyl-CoA, and wherein the production of isoprene is increased relative to cells that do not comprise the one or more heterologous nucleic acids encoding a polypeptide having phosphoketolase activity.
Recombinant microbial cells capable of producing isoprenoid precursors from a carbon source, wherein the cells comprise one or more heterologous nucleic acids encoding a polypeptide having phosphoketolase activity and one or more nucleic acids encoding one or more polypeptides of the complete MVA pathway, wherein culturing of said recombinant cells in a suitable media provides for the production of isoprenoid precursors, wherein the production of isoprenoid precursors occurs without an increase in intracellular acetyl-CoA, and wherein the production of isoprenoid precursors is increased relative to cells that do not comprise the one or more heterologous nucleic acids encoding a polypeptide having phosphoketolase activity.
Recombinant microbial cells capable of producing of an isoprenoid from a carbon source, wherein the cells comprise one or more heterologous nucleic acids encoding a polypeptide having phosphoketolase activity and (i) one or more nucleic acids encoding one or more polypeptides of the complete MVA pathway and (ii) a heterologous nucleic acid encoding a polyprenyl pyrophosphate synthase polypeptide, wherein culturing of said recombinant cells in a suitable media provides for the production of the isoprenoid, wherein the production the isoprenoid occurs without an increase in intracellular acetyl-CoA, and wherein the production of the isoprenoid is increased relative to cells that do not comprise the one or more heterologous nucleic acids encoding a polypeptide having phosphoketolase activity.
Dependencies are inferred from the claim text.