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US 8,993,252 B2Granted
Methods for detecting antibody that specifically bind a siderophore receptor polypeptide
AssigneeEpitopix, LLC
InventorsDaryll A. Emery, Darren E. Straub
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Abstract
The present invention provides compositions including siderophore receptor polypeptides and porins from gram negative microbes, and preferably, lipopolysaccharide at a concentration of no greater than about 10.0 endotoxin units per milliliter. The present invention also provides methods of making and methods of using such compositions.
Claims9
A method for detecting antibody comprising:
- contacting antibody with a preparation to result in a mixture, wherein the preparation comprises at least two siderophore receptor polypeptides isolated from a gram negative microbe and lipopolysaccharide at a concentration of no greater than about 10.0 endotoxin units per milliliter (EU/ml), wherein the at least two siderophore receptor polypeptides have molecular weights of between about 60 kDa and about 100 kDa as determined by separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis;
- incubating the mixture under conditions to allow the antibody to specifically bind a siderophore receptor polypeptide to form a siderophore receptor polypeptide:antibody complex;
- and
- detecting the siderophore receptor polypeptide:antibody complex, wherein detection of the siderophore receptor polypeptide:antibody complex indicates the presence of antibody that specifically binds a siderophore receptor polypeptide.
1 more element
Dependencies are inferred from the claim text.