Back to results
US 8,986,988 B2Granted
Cell rolling separation
AssigneeMassachusetts Institute of Technology
InventorsRohit Nandkumar Karnik, Seungpyo Hong, Ying Mei, Daniel Griffith Anderson, Jeffrey Michael Karp, Robert S. Langer, Suman Bose
Life of this patent
Estimating the term…
Abstract
The present invention provides systems for cell separation based on cell rolling on surfaces along edges of regions coated with cell adhesion molecules. A variety of designs of coated regions and edges are disclosed.
Claims68
A method comprising steps of:
- providing a surface that is at least partially coated with an ordered layer of cell adhesion molecules
- wherein the surface comprises at least one edge between an area coated with the ordered layer and another area that is not coated with the ordered layer;
- and
- wherein the area that is coated with the ordered layer of cell adhesion molecules is entirely covered with the ordered layer of cell adhesion molecules;
- and
- flowing a cell stream that contains a population of cells across the surface in a direction which forms a non-zero angle αs with the at least one edge, wherein at least one cell in the population of cells comprises a surface moiety that is recognized by the cell adhesion molecules and wherein the at least one cell rolls for a period of time in a direction that is αs to the direction of flow as a result of rolling along at least a portion of the at least one edge.
3 more elements
A method comprising steps of:
- providing a three dimensional surface that is at least partially coated with an ordered layer of cell adhesion molecules, and
- flowing a cell stream that contains a population of cells, simultaneously with a buffer stream that does not contain cells, across the surface in such conditions to create a stagnation line of no flow, wherein the direction of flow forms a non-zero angle αs with the stagnation line, wherein at least one cell in the population of cells comprises a surface moiety that is recognized by the cell adhesion molecules and wherein at least one cell in the population of cells rolls at least part of the time along the stagnation line in a direction that is αs to the direction of flow.
A method comprising steps of:
- providing a surface that is at least partially coated with an ordered layer of cell adhesion molecules, wherein the surface comprises at least one edge between an area coated with the ordered layer and another area that is not coated with the ordered layer;
- and
- flowing a first cell stream that contains a population of cells, simultaneously with a buffer stream that does not contain cells, across the surface in a direction which forms a non-zero angle αs with the at least one edge, wherein at least one cell in the population of cells comprises a surface moiety that is recognized by the cell adhesion molecules and wherein the at least one cell rolls for a period of time in a direction that is αs to the direction of flow as a result of rolling along at least a portion of the at least one edge.
Dependencies are inferred from the claim text.