Immobilized multi-layer artificial membrane for permeability measurements (PAMPA)
AssigneeCorning Incorporated
InventorsXiaoxi (Kevin) Chen, Charles L. Crespi
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Abstract
With the subject invention, a method is provided for preparing a filter membrane including the steps of dispersing a liquid which is generally hydrophobic into the pores of a porous membrane, and applying a solution containing lipids onto at least a first surface of the porous membrane containing the liquid. Advantageously, the subject invention allows for filter membranes to be prepared which can be stored for periods of time without degradation in performance. The subject invention may have applicability in various contexts, but is well-suited for preparing filter membranes for permeability screening, particularly Parallel Artificial Membrane Permeability Assay (PAMPA).
Claims21
A method of preparing a filter membrane comprising the sequential steps of:
- dispersing a hydrophobic liquid into pores of a porous filter membrane having a first and a second surface to form a continuous hydrophobic liquid layer across the porous filter membrane;
- then,
- applying a solution comprising phospholipids in an alkane-solvent, onto the first surface of said porous filter membrane containing the hydrophobic liquid so as to form a first phospholipid layer on said first surface, then,
- applying the solution onto the second surface of said porous filter membrane containing the hydrophobic liquid so as to form a second phospholipid layer on said second surface.
A method of preparing a filter membrane comprising the sequential steps of:
- dispersing a hydrophobic liquid into pores of a porous filter membrane comprising polyvinylidine difluoride or a polycarbonate having a first and a second surface to form a hydrophobic liquid layer therein;
- then,
- applying a solution comprising lipids in an alkane-solvent onto the first surface of the porous filter membrane containing the hydrophobic liquid to form a first lipid layer extending across the first surface.
Dependencies are inferred from the claim text.