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US 8,986,743 B2Granted
Animal model for the human immune system, and method for producing the same
AssigneeUniversitäT Leipzig
InventorsFrank Emmrich, Manja Kamprad, Manuela Ackermann
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Abstract
In a method for preparing an animal model for the human immune system in a non-human mammal, human stem cells with hematopoietic potential are transplanted into a non-human mammal. The non-human mammal is conditioned with cell culture supernatant of a culture of human cell lines, cells and/or tissue. The cell culture supernatant is derived from cell lines producing cytokines and other molecular mediators.
Claims8
A method for producing human immune-competent cells in a genetically immunodeficient mouse, wherein the mouse is a NOD mouse or a NOD/SCID mouse, the method comprising:
- a) transplanting an amount of human mononuclear cells (MNC) into the genetically immunodeficient mouse, and
- b) administering to the genetically immunodeficient mouse a conditioned cell culture supernatant of a culture of at least one member of the group consisting of human cell lines, cells and tissue;
- wherein the conditioned cell culture supernatant contains the factors:
- interleukin-8 (IL-8), interleukin-6 (IL-6), vascular endothelial growth factor (VEGF), interferon-γ-induced protein 10 (IP-10), monocyte chemoattractant protein-1 (MCP-1), angiogenin (ANG) and tissue-type plasminogen activator (t-PA) , each of the factors contained in a concentration of 20 pg/ml to 10 ng/ml, wherein 8 weeks after the transplantation of said MNC, said immunodeficient mouse produces T-cells and B-cells, monocytes, dendritic cells, NK cells, granulocytes, and CD34+ stem cells, wherein the amount of human MNC is sufficient to establish at least 29% T-cells and 20% B-cells of human origin in the spleen of the mouse.
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