Antibodies for the treatment of Clostridium difficile-associated infection and disease

An isolated antibody or an antigen-binding fragment thereof, wherein the antibody or antigen-binding fragment thereof:

• (a) specifically binds toxin A of C. difficile and the VH comprises the CDR1, CDR2, and CDR3 of SEQ ID NO:1 or SEQ ID NO:2 and the VL comprises the CDR1, CDR2, and CDR3 of the SEQ ID NO:3 or SEQ ID NO:4;
• or
• (b) specifically binds toxin A of C. difficile and the VH comprises the CDR1, CDR2, and CDR3 of SEQ ID NO:5 of SEQ ID NO:6 and the VL comprises the CDR1, CDR2, and CDR3 of SEQ ID NO:7;
• or
• (c) specifically binds toxin B of C. difficile and the VH comprises the CDR1, CDR2, and CDR3 of SEQ ID NO:8 or SEQ ID NO:9 and the VL comprises the CDR1, CDR2, and CDR3 of SEQ ID NO:10.

An isolated antibody or an antigen-binding fragment thereof, wherein the antibody or antigen-binding fragment thereof:

• (a) specifically binds toxin A of C. difficile, the VH comprises a CDR1 comprising amino acid residues 31 to 35 of SEQ ID NO:1, a CDR2 comprising amino acid residues 50 to 65 of SEQ ID NO:1 and a CDR3 comprising amino acid residues 95 to 102 of SEQ ID NO:1, and the VL comprises a CDR1 comprising amino acid residues 24 to 34 of SEQ ID NO:3, a CDR2 comprising amino acid residues 50 to 56 of SEQ ID NO:3, and a CDR3 comprising amino acid residues 89 to 97 of the SEQ ID NO:3;
• or
• (b) specifically binds toxin A of C. difficile, the VH comprises a CDR1 comprising amino acid residues 31 to 35 of SEQ ID NO:5, a CDR2 comprising amino acid residues 50 to 55 of SEQ ID NO:3, and a CDR3 comprising amino acid residues to of SEQ ID NO:
• 95 to 102 of SEQ ID NO:5, and the VL comprises a CDR1 comprising amino acid residues 24 to 33 of SEQ ID NO:7, a CDR2 comprising amino acid residues 49 to 55 of SEQ ID NO:7, and a CDR3 comprising amino acid residues 88 to 94 of SEQ ID NO:7;
• or
• (c) specifically binds toxin B of C. difficile, the VH comprises a CDR1 comprising amino acid residues 31 to 36 of SEQ ID NO:8, a CDR2 comprising amino acid residues 50 to 66 of SEQ ID NO:8, and a CDR3 comprising amino acid residues 99 to 108 of SEQ ID NO:8, and the VL comprises a CDR1 comprising amino acid residues 24 to 34 of SEQ ID NO:10, a CDR2 comprising amino acid residues 50 to 56 of SEQ ID NO:10, and a CDR3 comprising amino acid residues 89 to 97 of SEQ ID NO:10.