Somatic stem cells
AssigneeStemBios Technologies, Inc.
InventorJames Wang
Life of this patent
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Abstract
A method of treating a cellular proliferative disorder in a subject by obtaining from a subject a bodily fluid sample containing a plurality of cells, incubating the sample with EDTA or heparin in a container until the sample is separated into an upper layer and a lower layer, collecting the upper layer, isolating from the upper layer a population of somatic stem cells that are 0.3-6.0 micrometers in size, differentiating the somatic stem cells to dendritic cells in a medium containing GCSF, SCF, EGF, PDGF, bFGF, and IL-3, purifying the dendritic cells, contacting the dendritic cells thus purified with a cancer antigen, and administrating an effective amount of the dendritic cells presenting the cancer antigen to a subject in need thereof.
Claims15
A method of treating a cellular proliferative disorder in a subject, the method comprising:
- obtaining a bodily fluid sample containing a plurality of cells,
- incubating the sample with EDTA or heparin in a container until the sample is separated into an upper layer and a lower layer,
- collecting the upper layer,
- isolating from the upper layer a population of somatic stem cells that are 0.3-6.0 micrometers in size and CD9+,
- differentiating the somatic stem cells to dendritic cells in a medium containing GCSF, SCF, EGF, PDGF, bFGF, and IL-3, the dendritic cells being CD83+ and CD86+,
- purifying the dendritic cells,
- contacting the dendritic cells thus purified with a cancer antigen, and
- administering an effective amount of the dendritic cells presenting the cancer antigen to a subject in need thereof.
Dependencies are inferred from the claim text.