MNTF differentiation and growth of stem cells
AssigneeGenervon Biopharmaceuticals LLC
InventorsDeepa M. Deshpande, Douglas A. Kerr, Dorothy Pui-Yuk Ko
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Abstract
The present invention provides methods for inducing differentiation of an embryonic stem cell into a motor neuron using a motoneuronotrophic factor (MNTF) or its peptide analogs. The present invention further provides a method for isolating a population of stem cell derived motor neurons and a population of cells comprising the differentiated neural cells. Additionally, the present invention is directed to a method of enhancing the survival of the differentiated neural cells in long term cell cultures. Finally, the present invention provides compositions containing MNTF or its peptide analogs for therapeutic use in conjunction with stem cells.
Claims22
A method for differentiating mouse embryonic stem cells or mouse pluripotent stem cells, said method comprising i) obtaining a population of mouse embryonic stem cells or mouse pluripotent stem cells;
- ii) culturing the population of mouse embryonic stem cells or mouse pluripotent stem cells in a differentiating medium comprising a motoneuronotrophic factor (MNTF) analogue having the amino acid sequence of SEQ ID NO:1 or SEQ ID NO:4 and retinoic acid to induce differentiation into neurons;
- iii) inducing proliferation of the neurons;
- and, optionally iv) transplanting the neurons to said host.
A method for differentiating mouse embryonic stem cells or mouse pluripotent stem cells and transplanting the progeny to a host, said method comprising i) obtaining a population of mouse embryonic stem cells or mouse pluripotent stem cells;
- ii) culturing the population of mouse embryonic stem cells or mouse pluripotent stem cells in a differentiating medium until the population differentiates into embryoid bodies, iii) culturing the embryoid bodies in a culture medium comprising a motoneuronotrophic factor (MNTF) analogue having the amino acid sequence of SEQ ID NO:1 or SEQ ID NO:4 and retinoic acid to induce differentiation of the embryoid bodies into neurons;
- iv) inducing proliferation of the neurons;
- and, optionally) transplanting the neurons to said host.
Dependencies are inferred from the claim text.