Soft tissue and bone augmentation and bulking utilizing muscle-derived progenitor cells, compositions and treatments thereof

A method of restoring or improving contractility of a smooth muscle tissue in a mammal, comprising

• (a) isolating the skeletal muscle-derived progenitor cells (MDCs) according to a culture method comprising:
• (i) plating a suspension of cells from skeletal muscle tissue in a cell culture media in a first container, to which fibroblast cells in the muscle tissue suspension adhere;
• (ii) re-plating non-adherent cells in the cell culture media from step (i) in a second container containing media after approximately 15% to 20% of the cells from the cell suspension have adhered to the first container;
• (iii) re-plating the non-adherent cells in step (ii) at least one time in another cell culture container to enrich for an end population of viable, non-fibroblast, desmin-expressing said MDCs;
• and
• (iv) isolating said end population of viable, non-fibroblast, desmin-expressing MDCs from the container in step (iii);
• and
• (b) administering to said smooth muscle tissue a physiologically acceptable composition comprising isolated MDCs in an amount sufficient to restore or improve contractility of said smooth muscle, and a carrier, excipient or diluents, wherein the physiologically acceptable composition bulks, fills, or supports the smooth muscle tissue.