Methods and system for detecting soluble amyloid-β
AssigneeThe General Hospital Corporation
InventorsChongzhao Ran, Anna Moore
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Abstract
The present invention provides methods of detecting soluble amyloid β using curcumin-derived near infrared (NIR) imaging probes. Upon interacting with soluble amyloid β, these probes undergo a range of changes, qualifying them as “smart” probes. In addition, the invention provides methods of detecting soluble amyloid β by positron emission tomography (PET), magnetic resonance imaging (MRI) and multimodal imaging based on the curcumin-derived NIR imaging probes and derivatives thereof.
Claims15
A method for detecting soluble amyloid beta in a sample, comprising:
- (a) contacting a sample comprising soluble amyloid beta with a compound having the formula Ar1-L-Ar2, wherein
- L is a divalent linking group comprising an alkenylene having 5 to 15 backbone carbon atoms, wherein at least two of the backbone carbon atoms form part of a difluoroboronate ring, and Ar1 and Ar2 are each independently alkyl amine-substituted phenyl or pyridyl groups;
- wherein said compound binds soluble amyloid beta in the sample;
- (b) illuminating said compound bound to the soluble amyloid beta with near infrared light of a wavelength absorbable by said compound;
- and
- (c) detecting fluorescence emitted by the compound wherein said fluorescence corresponds to the soluble amyloid beta contained in said sample.
An in vivo optical imaging method for soluble amyloid beta detection in a subject, comprising:
- (a) administering to a subject a compound having the formula Ar1-L-Ar2, wherein
- L is a divalent linking group comprising an alkenylene having 5 to 15 backbone carbon atoms, wherein at least two of the backbone carbon atoms form part of a difluoroboronate ring, and Ar1 and Ar2 are each independently alkyl amine-substituted phenyl or pyridyl groups;
- wherein said compound binds soluble amyloid beta contained in the subject;
- (b) illuminating said compound bound to the soluble amyloid beta with near infrared light of a wavelength absorbable by said compound;
- and
- (c) detecting fluorescence emitted by the compound wherein said fluorescence corresponds to the soluble amyloid beta contained in said subject.
A method for providing an image of soluble amyloid beta contained within a subject by positron emission tomography (PET) scan, comprising:
- (a) administering to a subject a 11C or 18F labeled derivative of a compound having the formula Ar1-L-Ar2, wherein
- L is a divalent linking group comprising an alkenylene having 5 to 15 backbone carbon atoms, wherein at least two of the backbone carbon atoms form part of a difluoroboronate ring, and Ar1 and Ar2 are each independently alkyl amine-substituted phenyl or pyridyl groups;
- wherein said compound binds soluble amyloid beta contained in the subject;
- and
- (b) imaging gamma rays that are emitted due to the compound being bound to the soluble amyloid beta within said subject in order to provide an image of the soluble amyloid beta contained in said subject.
A method for providing an image of soluble amyloid beta contained within a subject by magnetic resonance imaging, comprising:
- (a) administering to a subject a 19F labeled derivative of a compound having the formula Ar1-L-Ar2, wherein
- L is a divalent linking group comprising an alkenylene having 5 to 15 backbone carbon atoms, wherein at least two of the backbone carbon atoms form part of a difluoroboronate ring, and Ar1 and Ar2 are each independently alkyl amine-substituted phenyl or pyridyl groups;
- wherein said compound binds soluble amyloid beta contained in the subject;
- and
- (b) imaging the subject in order to obtain a magnetic resonance image of the compound bound to the soluble amyloid beta and contained within the subject.
Dependencies are inferred from the claim text.