Loading patent…
AssigneesHirosaki University, National University Corporation Tokyo Unversity of Agriculture and Technology
InventorsKatsuya Yamada, Hideaki Matsuoka, Tadashi Teshima, Toshihiro Yamamoto
Estimating the term…
An object of the present invention is to provide a method for accurately evaluating the specific incorporation of D-glucose into cells. The present invention as a means for achieving the object is characterized by comprising contacting a D-glucose derivative that has a fluorescent chromophore in the molecule and is specifically incorporated into cells and an L-glucose derivative that has a fluorescent chromophore in the molecule with different cells in the same cell strain to be evaluated, respectively, comparing the fluorescence emitted by the D-glucose derivative that has a fluorescent chromophore in the molecule and is specifically incorporated into cells with the fluorescence emitted by the L-glucose derivative that has a fluorescent chromophore in the molecule, and evaluating the specific incorporation of D-glucose into cells relative to L-glucose by taking the difference between the two kinds of fluorescence intensities.
A method for evaluating the specific incorporation of D-glucose into cells relative to L-glucose, characterized by comprising contacting a D-glucose derivative that has a fluorescent chromophore in the molecule and an L-glucose derivative that has a fluorescent chromophore in the molecule with different cells in the same cell strain to be evaluated, respectively, comparing the fluorescence emitted by the D-glucose derivative with the fluorescence emitted by the L-glucose derivative, and evaluating the specific incorporation of D-glucose into cells relative to L-glucose by taking the difference between the two kinds of fluorescence intensities
A method for evaluating the specific incorporation of D-glucose into cells relative to L-glucose, characterized by comprising contacting a mixture of a D-glucose derivative that has a fluorescent chromophore in the molecule and one or more L-glucose derivatives that each have, in the molecule, a fluorescent chromophore that emits fluorescence at a wavelength different from that of the fluorescence emitted by the D-glucose derivative with cells to be evaluated, and detecting the fluorescence emitted by the D-glucose derivative and the fluorescence emitted by the L-glucose derivative, respectively,
Dependencies are inferred from the claim text.