Testing cartridge for an in vitro medical diagnostic device

A disposable assay cartridge comprising a housing having at least a top end and a bottom end,

• the top end further comprising an inlet, including an interface for accepting a receptacle containing a sample fluid;
• a sample fluid channel in fluid communication with the inlet for receiving sample fluid, the sample fluid channel being interrupted by a valve that controls a flow of sample fluid into an interior fluid channel that is in fluid communication with (i) a calibration fluid channel, (ii) an array comprising a plurality of electrochemical sensors, and (iii) a waste area downstream of the array comprising a plurality of electrochemical sensors for accepting spent fluids, including used calibration fluid;
• the bottom end further comprising a second inlet for introducing calibration fluid or air into the calibration fluid channel and an outlet for communication with pressure or vacuum pump for aspiration of calibration fluid, air, or sample fluid.