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US 8,986,282 B2Granted
Methods, compositions and apparatus for delivering heterologous molecules to cells
AssigneeThe Trustees of the University of Pennsylvania
InventorsHansell H. Stedman, Charles R. Bridges
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Abstract
A method for transferring a macromolecular complex to muscle cells by exsanguinating a region of the subject's microvasculature and delivering the complex to this region under high hydrostatic pressure. A balloon catheter having a balloon that extends substantially the full length of the cannula that is inserted into the subject is provided for use in the systemic delivery of macromolecular complex.
Claims18
A method for transferring a macromolecular complex to muscle cells, said method comprising the steps of:
- (a) isolating a region of the subject's microvasculature;
- and
- (b) delivering a macromolecular complex to the venous side of the isolated region of the subject's microvasculature under about 50 mm Hg to about 500 mm Hg hydrostatic pressure, wherein said hydrostatic pressure transfers said macromolecular complex to the muscle cells, wherein said macromolecular complex comprises a viral vector carrying a target molecule, wherein said viral vector is a recombinant adenoviral (rAd) vector or a recombinant adeno-associated viral (rAAV) vector,
- wherein said macromolecular complex is delivered substantially in the absence of vascular permeability-enhancing agents.
1 more element
Dependencies are inferred from the claim text.